• Vaccine Name: live attenuated Brucella suis vaccine expressing multimeric GnRH
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009414
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Pig
• Host Species as Laboratory Animal Model: Mus musculus
• Antigen: multimeric GnRH
• Preparation: The vaccine strain is leucine auxotrophic, allowing over-expression of heterologous antigens without antibiotic resistance markers. (Smith et al, 2019)
• Description: This study reports the development of a live-attenuated Brucella suis vaccine strain that is auxotrophic for leucine and capable of expressing heterologous antigens, specifically multimeric GnRH, aimed at controlling feral swine populations and brucellosis. Efficacy: The attenuated vaccine strain provided significant protection against Brucella suis challenge in mice. Immune response: Vaccinated mice developed antibodies against the expressed multimeric GnRH antigen. (Smith et al, 2019)

Mouse Response

• Immune Response: Vaccinated mice developed antibodies against the expressed multimeric GnRH antigen. (Smith et al, 2019)
• Challenge Protocol: Protection against Brucella suis challenge was evaluated in mice; antibody development against the expressed antigen was also assessed. (Smith et al, 2019)
• Efficacy: The attenuated vaccine strain provided significant protection against Brucella suis challenge in mice. (Smith et al, 2019)
• Description: Protection against Brucella suis challenge was evaluated in mice; antibody development against the expressed antigen was also assessed. (Smith et al, 2019)