• Vaccine Name: Brucella abortus BCSP31 DNA vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009407
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: rabbits (Oryctolagus cuniculus)
• Antigen: BCSP31
• Preparation: The BCSP31 gene was cloned into pTZ57 R/T vector, subcloned into pET-28a vector, expressed in E. coli BL21, and the purified protein was used for immunization. (Imtiaz et al, 2018)
• Description: The study aimed to construct a DNA vaccine encoding the antigenic surface protein BCSP31 of Brucella abortus to control brucellosis, a highly contagious zoonotic disease with high prevalence in Pakistan. Efficacy: The vaccinated groups showed significantly higher IgG titers in a dose-dependent manner compared to control. The DNA vaccine provided a log unit protection of 3.02 against Brucella abortus challenge in rabbits. Immune response: The vaccine induced a significant specific IgG antibody response against BCSP31 antigen in vaccinated rabbits, indicating immunogenicity and potential protective immunity. (Imtiaz et al, 2018)

Rabbit Response

• Immune Response: The vaccine induced a significant specific IgG antibody response against BCSP31 antigen in vaccinated rabbits, indicating immunogenicity and potential protective immunity. (Imtiaz et al, 2018)
• Efficacy: The vaccinated groups showed significantly higher IgG titers in a dose-dependent manner compared to control. The DNA vaccine provided a log unit protection of 3.02 against Brucella abortus challenge in rabbits. (Imtiaz et al, 2018)
• Description: Immunogenic and protective efficacy was evaluated in rabbits divided into four groups; three groups received different doses (750, 1500, 3000 μg/0.2 mL) of purified protein via intraconjunctival route, and one control group received saline. IgG responses were measured weekly by indirect ELISA. (Imtiaz et al, 2018)