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Vaccine Detail
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live attenuated Brucella melitensis strain M5-90 manB mutant vaccine |
| Vaccine Information |
- Vaccine Name: live attenuated Brucella melitensis strain M5-90 manB mutant vaccine
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009383
- Type: Live attenuated vaccine
- Status: Research
- Host Species for Licensed Use: Goat
- Host Species as Laboratory Animal Model: BALB/c mice
- Antigen: manB mutant
- Preparation: Live attenuated Brucella melitensis M5-90 strain with manB gene deletion. (Zhang et al, 2017)
- Description: The vaccine candidate M5-90ΔmanB was generated to overcome drawbacks of current vaccines such as residual virulence and inability to differentiate infection from vaccination. Efficacy: M5-90ΔmanB showed significantly reduced survival in macrophages and mice and conferred strong protective immunity against virulent Brucella melitensis 16M challenge in BALB/c mice. Immune response: Induced anti-Brucella-specific IgG1 and IgG2a responses; secretion of IFN-γ and IL-4 in mice; IFN-γ secretion in goats; goats inoculated with M5-90ΔmanB were seronegative by RBPT and STAT, allowing differentiation from natural infection. (Zhang et al, 2017)
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| Host Response |
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| References |
Zhang et al, 2017: Zhang J, Yin S, Yi D, Zhang H, Li Z, Guo F, Chen C, Fang W, Wang J. The Brucella melitensis M5-90ΔmanB live vaccine candidate is safer than M5-90 and confers protection against wild-type challenge in BALB/c mice. Microbial pathogenesis. 2017 Nov; 112; 148-155. [PubMed: 28916316].
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