• Vaccine Name: live attenuated Brucella abortus strain 2308 delta NodV delta NodW vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009375
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus; RAW 264.7 murine macrophages; BALB/c mice
• Antigen: NodV and NodW (two-component regulatory system genes deleted)
• Preparation: Live attenuated double mutant strain 2308 with deletions in NodV and NodW genes; no adjuvant reported. (Li et al, 2017)
• Description: The vaccine is a double deletion mutant of virulent Brucella abortus strain 2308, deleting the NodV and NodW genes encoding a two-component regulatory system on chromosome II, constructed to reduce virulence and allow serological differentiation from infected animals. Efficacy: The mutant showed significantly reduced survival in macrophages and mice, no splenomegaly in inoculated mice, and conferred high protective immunity against challenge with wild-type strain 2308 in BALB/c mice. Immune response: Induced anti-Brucella-specific IgG antibody response; stimulated secretion of gamma interferon (IFN-γ) and interleukin-4 (IL-4), indicating activation of both Th1 and Th2 immune responses. (Li et al, 2017)

Mouse Response

• Host Strain: BALB/c
• Immune Response: Induced anti-Brucella-specific IgG antibody response; stimulated secretion of gamma interferon (IFN-γ) and interleukin-4 (IL-4), indicating activation of both Th1 and Th2 immune responses. (Li et al, 2017)
• Challenge Protocol: Survival assays in murine macrophage cell line RAW 264.7; infection and protection studies in BALB/c mice including challenge with wild-type strain 2308; measurement of splenomegaly; serological assays for IgG; cytokine secretion assays for IFN-γ and IL-4. (Li et al, 2017)
• Efficacy: The mutant showed significantly reduced survival in macrophages and mice, no splenomegaly in inoculated mice, and conferred high protective immunity against challenge with wild-type strain 2308 in BALB/c mice. (Li et al, 2017)
• Description: Survival assays in murine macrophage cell line RAW 264.7; infection and protection studies in BALB/c mice including challenge with wild-type strain 2308; measurement of splenomegaly; serological assays for IgG; cytokine secretion assays for IFN-γ and IL-4. (Li et al, 2017)