• Vaccine Name: live attenuated Brucella suis delta pgm vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009358
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Pig
• Host Species as Laboratory Animal Model: Mus musculus; cultured cells
• Antigen: pgm gene deletion
• Preparation: Live attenuated strain with clean deletion of the pgm gene. (Czibener et al, 2016)
• Description: A novel vaccine strain was constructed by deleting the phosphoglucomutase (pgm) gene, which is involved in polysaccharide biosynthesis. This strain lacks complete lipopolysaccharide, cannot synthesize cyclic beta glucans, and is sensitive to detergents and Polymyxin B. Efficacy: The Delta-pgm strain was completely avirulent in mice and conferred excellent protection against challenge with virulent Brucella suis. Immune response: Vaccination induced the production of pro-inflammatory cytokines. (Czibener et al, 2016)

Mouse Response

• Immune Response: Vaccination induced the production of pro-inflammatory cytokines. (Czibener et al, 2016)
• Efficacy: The Delta-pgm strain was completely avirulent in mice and conferred excellent protection against challenge with virulent Brucella suis. (Czibener et al, 2016)
• Description: Replication was tested in cultured cells; virulence and protection were evaluated in a mouse model of infection. (Czibener et al, 2016)