• Vaccine Name: live attenuated Brucella abortus strain BA15 cydC purD double deletion vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009349
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus; murine macrophages; BALB/c mice
• Antigen: cydC; purD
• Preparation: Live double deletion mutant without adjuvant. (Truong et al, 2016)
• Description: Constructed from virulent Brucella abortus biovar 1 field isolate (BA15) by deleting genes encoding ATP-binding cassette-type transporter (cydC) and phosphoribosylamine-glycine ligase (purD). Efficacy: Highly attenuated; cleared from spleens by 4 weeks post-inoculation; no splenomegaly; induced long-term protective immunity; provided significantly better protection than commercial Brucella abortus strain RB51 vaccine. Immune response: Induced humoral and cell-mediated immune responses in mice. (Truong et al, 2016)

Mouse Response

• Host Strain: BALB/c
• Host cell type: macrophages
• Immune Response: Induced humoral and cell-mediated immune responses in mice. (Truong et al, 2016)
• Challenge Protocol: Virulence assays in murine macrophages and BALB/c mice; clearance from spleens; immune response and protection against challenge with B. abortus strain 2308. (Truong et al, 2016)
• Efficacy: Highly attenuated; cleared from spleens by 4 weeks post-inoculation; no splenomegaly; induced long-term protective immunity; provided significantly better protection than commercial Brucella abortus strain RB51 vaccine. (Truong et al, 2016)
• Description: Virulence assays in murine macrophages and BALB/c mice; clearance from spleens; immune response and protection against challenge with B. abortus strain 2308. (Truong et al, 2016)