• Vaccine Name: live attenuated Brucella abortus strain RB51 cydC deletion vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009339
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice); murine macrophages
• Antigen: cydC deletion; cydD deletion; purD deletion; cydC cydD double deletion; cydC purD double deletion
• Preparation: Live attenuated RB51 mutants with single deletions (ΔcydC, ΔcydD, ΔpurD) and double deletions (ΔcydCΔcydD, ΔcydCΔpurD) were generated. (Truong et al, 2015a)
• Description: Brucella abortus attenuated strain RB51 vaccine is widely used for prevention of bovine brucellosis but has drawbacks including residual virulence; gene deletion mutants of RB51 were constructed to increase safety. Efficacy: Single immunization with mutants induced lower protection than parental RB51; booster immunization with mutants provided significant protection against both homologous B. abortus and heterologous B. canis challenge. Immune response: Mutants induced a mixed but T-helper-1-biased humoral and cellular immune response in immunized mice. (Truong et al, 2015a)

Mouse Response

• Host Strain: BALB/c
• Host cell type: macrophages
• Immune Response: Mutants induced a mixed but T-helper-1-biased humoral and cellular immune response in immunized mice. (Truong et al, 2015a)
• Challenge Protocol: In vitro virulence assays in murine macrophages; in vivo immunization and challenge studies in BALB/c mice with virulent B. abortus strain 2308 and Brucella canis strain 26. (Truong et al, 2015a)
• Efficacy: Single immunization with mutants induced lower protection than parental RB51; booster immunization with mutants provided significant protection against both homologous B. abortus and heterologous B. canis challenge. (Truong et al, 2015a)
• Description: In vitro virulence assays in murine macrophages; in vivo immunization and challenge studies in BALB/c mice with virulent B. abortus strain 2308 and Brucella canis strain 26. (Truong et al, 2015a)