• Vaccine Name: live attenuated Brucella ovis strain delta abcBA vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009328
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c and C57BL/6 mice)
• Antigen: abcBA deletion mutant
• Preparation: The vaccine was formulated as an encapsulated live attenuated ΔabcBA strain; both single and multiple doses were tested, with encapsulation significantly improving protection. (Silva et al, 2015)
• Description: The study evaluated the Brucella ovis ΔabcBA strain as a vaccine candidate in mice to assess protection against wild-type B. ovis challenge. Efficacy: Single or multiple immunizations with non-encapsulated strain provided mild protection with reduced liver bacterial loads but not spleen; encapsulated vaccine significantly improved protection in both mouse strains and prevented lesions in spleen and liver. (Silva et al, 2015)

Mouse Response

• Vaccination Protocol: BALB/c and C57BL/6 mice were immunized subcutaneously or intraperitoneally with single or multiple doses of the vaccine strain. (Silva et al, 2015)
• Challenge Protocol: BALB/c and C57BL/6 mice were immunized subcutaneously or intraperitoneally with single or multiple doses of the vaccine strain, then challenged with wild-type B. ovis; bacterial counts and lesion development in liver and spleen were assessed. (Silva et al, 2015)
• Efficacy: Single or multiple immunizations with non-encapsulated strain provided mild protection with reduced liver bacterial loads but not spleen; encapsulated vaccine significantly improved protection in both mouse strains and prevented lesions in spleen and liver. (Silva et al, 2015)
• Description: BALB/c and C57BL/6 mice were immunized subcutaneously or intraperitoneally with single or multiple doses of the vaccine strain, then challenged with wild-type B. ovis; bacterial counts and lesion development in liver and spleen were assessed. (Silva et al, 2015)