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Vaccine Detail
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B. melitensis vaccine strain M5-90ΔwboA |
| Vaccine Information |
- Vaccine Name: B. melitensis vaccine strain M5-90ΔwboA
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009326
- Type: Live attenuated vaccine
- Status: Research
- Host Species for Licensed Use: Sheep
- Host Species as Laboratory Animal Model: Mus musculus; RAW 264.7 murine macrophages
- Antigen: wboA deletion mutant
- Preparation: Live attenuated wboA deletion mutant of B. melitensis strain M5-90. (Li et al, 2015)
- Description: A wboA mutant was derived from Brucella melitensis vaccine strain M5-90 to address drawbacks of current vaccines including inability to serologically differentiate vaccinated from infected animals. Efficacy: The M5-90ΔwboA strain showed reduced survival in macrophages and mice, induced protective immunity comparable to parental M5-90 strain in mice, and elicited specific IgG and cytokine responses; in sheep, it induced IFN-γ secretion and did not cause positive results in standard serological tests. Immune response: Induced anti-Brucella-specific IgG, secretion of IFN-γ and IL-2 in mice; IFN-γ secretion in sheep; T-cell responses (CD4+, CD8+) observed; allowed serological differentiation between natural infection and vaccination using WboA antigen probes. (Li et al, 2015)
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| Host Response |
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| References |
Li et al, 2015: Li ZQ, Shi JX, Fu WD, Zhang Y, Zhang J, Wang Z, Li TS, Chen CF, Guo F, Zhang H. A Brucella melitensis M5-90 wboA deletion strain is attenuated and enhances vaccine efficacy. Molecular immunology. 2015 Aug; 66(2); 276-83. [PubMed: 25899866].
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