• Vaccine Name: live attenuated Brucella ovis Omp22 deletion vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009299
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Host Species as Laboratory Animal Model: Mus musculus
• Antigen: omp22 deletion
• Description: Two previously characterized B. ovis attenuated mutants including Δomp22 were evaluated as homologous vaccines for specific prophylaxis of B. ovis infection. Efficacy: Protective efficacy against B. ovis similar to that observed with B. melitensis Rev 1 vaccine. Immune response: Induced higher levels of anti-B. ovis IgG1, IgG2a, and IgG2b antibodies than Rev 1; splenocytes secreted similar TNF-α, IL12(p40), and high IFN-γ; higher IL-1α levels than Rev 1; lower IL-10 levels than Rev 1. (Sancho et al, 2014)

Mouse Response

• Immune Response: Induced higher levels of anti-B. ovis IgG1, IgG2a, and IgG2b antibodies than Rev 1; splenocytes secreted similar TNF-α, IL12(p40), and high IFN-γ; higher IL-1α levels than Rev 1; lower IL-10 levels than Rev 1. (Sancho et al, 2014)
• Efficacy: Protective efficacy against B. ovis similar to that observed with B. melitensis Rev 1 vaccine. (Sancho et al, 2014)
• Description: Vaccination of mice followed by measurement of antibody subclasses, cytokine secretion by splenocytes after antigen stimulation, assessment of persistence, splenomegaly, and protective efficacy against B. ovis. (Sancho et al, 2014)