• Vaccine Name: Brucella abortus L7/L12 Omp16 recombinant vector vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009294
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Antigen: L7/L12; Omp16
• Adjuvant: Montanide Gel01; chitosan
• Preparation: A bivalent vaccine formulation composed of a mixture of recombinant influenza A subtype H5N1 and H1N1 viruses expressing Brucella L7/L12 and Omp16 proteins, combined with adjuvants Montanide Gel01 or chitosan to increase effectiveness. (Tabynov et al, 2014)
• Description: This study presents a new candidate vector vaccine against Brucella abortus consisting of recombinant influenza A viruses expressing Brucella L7/L12 and Omp16 proteins. Efficacy: The vaccine provided a high level of protectiveness against B. abortus infection comparable to the commercial B. abortus strain 19 vaccine and superior to the strain 19 vaccine combined with Montanide Gel01 adjuvant. Immune response: Induced strong antigen-specific T-cell immune response with high numbers of CD4(+) and CD8(+) cells, increased IFN-γ concentration, and promoted formation of IgG antibodies predominantly of isotype IgG2a against Brucella L7/L12 and Omp16 proteins. (Tabynov et al, 2014)

Cattle Response

• Vaccination Protocol: Immunization of cattle (heifers aged 1-1.5 years, 5 animals per group) via conjunctival method using a cross prime (H5N1) and booster (H1N1) vaccination schedule at 28-day intervals; comparison with positive control (B. abortus S19) and negative control (PBS). (Tabynov et al, 2014)
• Immune Response: Induced strong antigen-specific T-cell immune response with high numbers of CD4(+) and CD8(+) cells, increased IFN-γ concentration, and promoted formation of IgG antibodies predominantly of isotype IgG2a against Brucella L7/L12 and Omp16 proteins. (Tabynov et al, 2014)
• Efficacy: The vaccine provided a high level of protectiveness against B. abortus infection comparable to the commercial B. abortus strain 19 vaccine and superior to the strain 19 vaccine combined with Montanide Gel01 adjuvant. (Tabynov et al, 2014)
• Description: Immunization of cattle (heifers aged 1-1.5 years, 5 animals per group) via conjunctival method using a cross prime (H5N1) and booster (H1N1) vaccination schedule at 28-day intervals; comparison with positive control (B. abortus S19) and negative control (PBS). (Tabynov et al, 2014)