• Vaccine Name: live attenuated Brucella abortus betB deletion mutant vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009289
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: HeLa cells; RAW 264.7 macrophages; Mus musculus
• Antigen: betB deletion mutant
• Preparation: Live betB deletion mutant strain of Brucella abortus. (Lee et al, 2014)
• Description: The study investigated a betB deletion mutant constructed from virulent Brucella abortus 544 as a potential vaccine candidate. Efficacy: The betB mutant was predominantly cleared from mouse spleens after 2 weeks post-infection and vaccination with the live betB mutant provided effective protection against challenge with the virulent wild-type strain. Immune response: The betB mutant showed increased adherence to host cells and enhanced phosphorylation of protein kinases promoting phagocytic activity; intracellular trafficking showed elevated colocalization with LAMP-1, indicating altered phagocytic pathway engagement. (Lee et al, 2014)

Mouse Response

• Immune Response: The betB mutant showed increased adherence to host cells and enhanced phosphorylation of protein kinases promoting phagocytic activity; intracellular trafficking showed elevated colocalization with LAMP-1, indicating altered phagocytic pathway engagement. (Lee et al, 2014)
• Challenge Protocol: In vitro stress resistance tests (osmotic and acid stress), internalization and replication assays in HeLa cells and RAW 264.7 macrophages, intracellular trafficking analysis with LAMP-1 colocalization, and in vivo mouse infection and vaccination challenge experiments. (Lee et al, 2014)
• Efficacy: The betB mutant was predominantly cleared from mouse spleens after 2 weeks post-infection and vaccination with the live betB mutant provided effective protection against challenge with the virulent wild-type strain. (Lee et al, 2014)
• Description: In vitro stress resistance tests (osmotic and acid stress), internalization and replication assays in HeLa cells and RAW 264.7 macrophages, intracellular trafficking analysis with LAMP-1 colocalization, and in vivo mouse infection and vaccination challenge experiments. (Lee et al, 2014)