• Vaccine Name: live attenuated Brucella suis strain 353-1 vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009283
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Pig
• Host Species as Laboratory Animal Model: Sus scrofa (domestic swine)
• Antigen: rough mutant of strain 353-1
• Preparation: Live natural rough mutant strain 353-1 administered by conjunctival or intramuscular routes at doses of 5 × 10^7 CFU or 1-2 × 10^10 CFU. (Stoffregen et al, 2013)
• Description: The study evaluated the safety, immunogenicity, and clearance of the natural rough mutant of Brucella suis strain 353-1 as a vaccine in domestic swine. Efficacy: The vaccine strain was nonpathogenic, cleared from most vaccinates by 10-12 weeks, did not cause significant tissue lesions, was phenotypically stable, and was not shed to sentinel animals. Immune response: Vaccination induced significant humoral responses, peripheral blood mononuclear cell proliferation, and interferon-gamma production compared to controls. (Stoffregen et al, 2013)

Pig Response

• Immune Response: Vaccination induced significant humoral responses, peripheral blood mononuclear cell proliferation, and interferon-gamma production compared to controls. (Stoffregen et al, 2013)
• Efficacy: The vaccine strain was nonpathogenic, cleared from most vaccinates by 10-12 weeks, did not cause significant tissue lesions, was phenotypically stable, and was not shed to sentinel animals. (Stoffregen et al, 2013)
• Description: Three studies with 105 pigs inoculated by conjunctival or intramuscular routes; periodic blood cultures, tissue collection at necropsy, histologic evaluation, serologic and microbiologic testing of co-housed sentinel animals. (Stoffregen et al, 2013)