• Vaccine Name: live attenuated Brucella melitensis strain NI-NB6 vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009274
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Host Species as Laboratory Animal Model: Mus musculus
• Antigen: wboA gene deletion
• Preparation: Live attenuated rough mutant with wboA gene deletion. (Wang et al, 2013)
• Description: Constructed as a rough-mutant strain with wboA gene deletion from Brucella melitensis NI parent strain to evaluate effects of genetic background on virulence, pathogenicity, and protective immunity. Efficacy: Showed longer survival time in mice (11 weeks) but offered less protection against challenge compared to some other mutants. Immune response: Induced protective immunity less effective than some other mutants; survival time in vivo not directly associated with protective immunity. (Wang et al, 2013)

Mouse Response

• Immune Response: Induced protective immunity less effective than some other mutants; survival time in vivo not directly associated with protective immunity. (Wang et al, 2013)
• Challenge Protocol: Survival and virulence assays in mice; protection tests against Brucella abortus 2308 challenge in mice. (Wang et al, 2013)
• Efficacy: Showed longer survival time in mice (11 weeks) but offered less protection against challenge compared to some other mutants. (Wang et al, 2013)
• Description: Survival and virulence assays in mice; protection tests against Brucella abortus 2308 challenge in mice. (Wang et al, 2013)