• Vaccine Name: live attenuated Brucella abortus strain RB51-leuB vaccine over-expressing SOD and WboA
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009237
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Pig
• Host Species as Laboratory Animal Model: Mus musculus
• Antigen: Cu/Zn superoxide dismutase (SOD); L7/L12 ribosomal protein; glycosyl-transferase (WboA)
• Preparation: Strain RB51leuB was used as a platform with an antibiotic-resistance marker free plasmid to construct strains over-expressing SOD alone, SOD and L7/L12, or SOD and WboA. (Rajasekaran et al, 2011)
• Description: An improved recombinant Brucella abortus strain RB51 leucine auxotroph (strain RB51leuB) was developed to over-express homologous proteins SOD, L7/L12, and WboA to protect against Brucella suis infection. Efficacy: All vaccine groups significantly protected mice compared to controls (P<0.05); the strain RB51leuB/SOD/WboA vaccine provided significantly better protection than strains RB51leuB/SOD or RB51leuB/SOD/L7/L12. Immune response: Over-expression of Brucella antigens in strain RB51leuB elicited protective immune responses against brucellosis. (Rajasekaran et al, 2011)

Mouse Response

• Immune Response: Over-expression of Brucella antigens in strain RB51leuB elicited protective immune responses against brucellosis. (Rajasekaran et al, 2011)
• Challenge Protocol: Protection efficacy was evaluated in a mouse model challenged with virulent Brucella suis. (Rajasekaran et al, 2011)
• Efficacy: All vaccine groups significantly protected mice compared to controls (P<0.05); the strain RB51leuB/SOD/WboA vaccine provided significantly better protection than strains RB51leuB/SOD or RB51leuB/SOD/L7/L12. (Rajasekaran et al, 2011)
• Description: Protection efficacy was evaluated in a mouse model challenged with virulent Brucella suis. (Rajasekaran et al, 2011)