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Vaccine Detail
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live attenuated Brucella abortus strain 19 vaccine expressing Mycobacterium bovis MPB83 fusion proteins |
| Vaccine Information |
- Vaccine Name: live attenuated Brucella abortus strain 19 vaccine expressing Mycobacterium bovis MPB83 fusion proteins
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009228
- Type: Live attenuated vaccine
- Status: Research
- Host Species for Licensed Use: Cattle
- Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice)
- Antigen: MPB83; fusion partners β-galactosidase, OMP19, BP26
- Preparation: Recombinant B. abortus S19 strains carrying genes coding for MPB83 fused to β-galactosidase, OMP19, or BP26 expressed from replicative or integrative plasmids; OMP19-MPB83 processed as a lipoprotein. (Sabio et al, 2010)
- Description: The immunodominant MPB83 antigen from Mycobacterium bovis was expressed as chimeric fusion proteins with β-galactosidase, outer membrane lipoprotein OMP19, or periplasmic protein BP26 in Brucella abortus strain 19, driven by each gene's own promoter. Efficacy: Recombinant S19 strains induced specific INF-γ production against MPB83; fusion to BP26 resulted in weaker immune response; no effect on bacterial survival or infection pattern. Immune response: Specific cellular immune response characterized by INF-γ production against MPB83 antigen; lipid moiety association (OMP19 fusion) did not enhance immunogenicity compared to other fusions. (Sabio et al, 2010)
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| Host Response |
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Mouse Response
- Host Strain: BALB/c
- Immune Response: Specific cellular immune response characterized by INF-γ production against MPB83 antigen; lipid moiety association (OMP19 fusion) did not enhance immunogenicity compared to other fusions. (Sabio et al, 2010)
- Efficacy: Recombinant S19 strains induced specific INF-γ production against MPB83; fusion to BP26 resulted in weaker immune response; no effect on bacterial survival or infection pattern. (Sabio et al, 2010)
- Description: Immunization of BALB/c mice with recombinant S19 strains; measurement of splenocyte INF-γ production in response to MPB83 stimulation; assessment of bacterial survival and infection pattern. (Sabio et al, 2010)
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| References |
Sabio et al, 2010: Sabio y García JV, Bigi F, Rossetti O, Campos E. Expression of MPB83 from Mycobacterium bovis in Brucella abortus S19 induces specific cellular immune response against the recombinant antigen in BALB/c mice. Microbes and infection. 2010 Dec; 12(14-15); 1236-43. [PubMed: 20888425].
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