• Vaccine Name: live attenuated Brucella abortus wbkA mutant vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009176
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice)
• Antigen: wbkA mutation
• Preparation: Live wbkA mutant cells used alone or in combination with live bvrS mutant, inactivated bvrS cells, or purified S-LPS. (Grilló et al, 2006)
• Description: The rough wbkA mutant is attenuated and previously shown to be a better vaccine than RB51 in mice. Efficacy: The wbkA mutant alone protected against virulent B. abortus but less effectively than when combined with the bvrS mutant; combination provided better protection than B. abortus strain 19 vaccine. Immune response: The combination with bvrS mutant induced an antibody response reduced compared to B. abortus strain 19 vaccination. (Grilló et al, 2006)

Mouse Response

• Host Strain: BALB/c
• Immune Response: The combination with bvrS mutant induced an antibody response reduced compared to B. abortus strain 19 vaccination. (Grilló et al, 2006)
• Challenge Protocol: Protection against virulent Brucella abortus challenge in BALB/c mice; measurement of splenic persistence and antibody response. (Grilló et al, 2006)
• Efficacy: The wbkA mutant alone protected against virulent B. abortus but less effectively than when combined with the bvrS mutant; combination provided better protection than B. abortus strain 19 vaccine. (Grilló et al, 2006)
• Description: Protection against virulent Brucella abortus challenge in BALB/c mice; measurement of splenic persistence and antibody response. (Grilló et al, 2006)