• Vaccine Name: inactivated Brucella abortus strain RB51 recombinant gamma-irradiated vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009174
• Type: Inactivated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice); dendritic cells (in vitro)
• Antigen: Escherichia coli beta-galactosidase (heterologous antigen)
• Preparation: Gamma-irradiated recombinant RB51 strain expressing beta-galactosidase; irradiated at minimum 300 kilorads to abolish replication but maintain metabolic activity and antigen synthesis. (Sanakkayala et al, 2005)
• Description: Brucella abortus strain RB51 is an attenuated rough mutant used as a live vaccine against bovine brucellosis. A recombinant RB51 strain expressing E. coli beta-galactosidase was gamma-irradiated to eliminate replicative ability while retaining metabolic activity and antigen expression. Efficacy: Gamma-irradiated recombinant RB51 induced significant protection against challenge with virulent Brucella abortus in mice, whereas heat-killed vaccine did not. Immune response: Induction of beta-galactosidase-specific Th1-type immune responses; gamma-irradiated vaccine stimulated dendritic cell maturation with higher interleukin-12 secretion compared to heat-killed vaccine; no differences in immune responses to B. abortus-specific antigens between groups. (Sanakkayala et al, 2005)

Mouse Response

• Host Strain: BALB/c
• Host cell type: dendritic cells
• Immune Response: Induction of beta-galactosidase-specific Th1-type immune responses; gamma-irradiated vaccine stimulated dendritic cell maturation with higher interleukin-12 secretion compared to heat-killed vaccine; no differences in immune responses to B. abortus-specific antigens between groups. (Sanakkayala et al, 2005)
• Efficacy: Gamma-irradiated recombinant RB51 induced significant protection against challenge with virulent Brucella abortus in mice, whereas heat-killed vaccine did not. (Sanakkayala et al, 2005)
• Description: Intraperitoneal inoculation of BALB/c mice with 10^9 CFU equivalents of gamma-irradiated recombinant RB51; comparison with heat-killed RB51; in vitro dendritic cell maturation and cytokine secretion assays. (Sanakkayala et al, 2005)