• Vaccine Name: Brucella ovis outer membrane complex microparticle subunit vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009173
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Antigen: outer membrane complex
• Preparation: Outer membrane complex antigen from Brucella ovis encapsulated in poly-epsilon-caprolactone microparticles prepared by a double emulsion solvent evaporation method (TROMS), with antigen loading about 13 microg HS per mg microparticles. (Muñoz et al, 2006)
• Description: A safer vaccine alternative to Brucella melitensis Rev. 1 was developed using an outer membrane complex from Brucella ovis encapsulated in poly-epsilon-caprolactone microparticles. Efficacy: Vaccination with 800 microg HS-PEC microparticles induced adequate serological response and conferred protection against Brucella ovis challenge similar to that of the live attenuated Rev. 1 vaccine; lower dose (80 microg) was less effective; revaccination increased serological response but not protection. Immune response: Adequate serological response against Brucella ovis antigens induced by 800 microg HS-PEC; no positive serological reactions in Rose Bengal and Complement Fixation tests in HS-PEC vaccinated animals, unlike Rev. 1 vaccinated animals which were seropositive in these tests. (Muñoz et al, 2006)

Sheep Response

• Vaccination Protocol: Subcutaneous vaccination of rams with 800 microg HS in PEC microparticles; comparison with Brucella melitensis strain Rev. 1 vaccine; serological tests (Rose Bengal and Complement Fixation tests). (Muñoz et al, 2006)
• Immune Response: Adequate serological response against Brucella ovis antigens induced by 800 microg HS-PEC; no positive serological reactions in Rose Bengal and Complement Fixation tests in HS-PEC vaccinated animals, unlike Rev. 1 vaccinated animals which were seropositive in these tests. (Muñoz et al, 2006)
• Challenge Protocol: Subcutaneous vaccination of rams with 800 microg HS in PEC microparticles; comparison with Brucella melitensis strain Rev. 1 vaccine; serological tests (Rose Bengal and Complement Fixation tests); challenge with Brucella ovis. (Muñoz et al, 2006)
• Efficacy: Vaccination with 800 microg HS-PEC microparticles induced adequate serological response and conferred protection against Brucella ovis challenge similar to that of the live attenuated Rev. 1 vaccine; lower dose (80 microg) was less effective; revaccination increased serological response but not protection. (Muñoz et al, 2006)
• Description: Subcutaneous vaccination of rams with 800 microg HS in PEC microparticles; comparison with Brucella melitensis strain Rev. 1 vaccine; serological tests (Rose Bengal and Complement Fixation tests); challenge with Brucella ovis. (Muñoz et al, 2006)