• Vaccine Name: Brucella abortus soluble protein extract (SPEBA) vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009170
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Bos taurus; bovine monocyte-derived macrophages
• Antigen: soluble protein extract of Brucella abortus (SPEBA)
• Adjuvant: recombinant bovine interleukin 2
• Preparation: Subunit vaccine consisting of 2 mg soluble protein extract administered with or without recombinant bovine IL 2. (Wyckoff et al, 2005)
• Description: The study evaluated immunization of cattle with a Brucella abortus soluble protein extract vaccine augmented by recombinant bovine IL 2. Efficacy: SPEBA vaccine alone provided limited protection; co-administration with rBoIL 2 improved antibody responses and lymphoproliferation but protection against abortion was not as strong as with S19 plus rBoIL 2. Immune response: Antibody responses were significantly higher in vaccinates receiving rBoIL 2; lymphoproliferation responses were higher in vaccinated or rBoIL 2-treated cattle; rBoIL 2 stimulated activation of macrophages with up-regulation of TNF-alpha, IL-12p40, and interferon-gamma genes. (Wyckoff et al, 2005)

Cattle Response

• Host cell type: macrophages
• Immune Response: Antibody responses were significantly higher in vaccinates receiving rBoIL 2; lymphoproliferation responses were higher in vaccinated or rBoIL 2-treated cattle; rBoIL 2 stimulated activation of macrophages with up-regulation of TNF-alpha, IL-12p40, and interferon-gamma genes. (Wyckoff et al, 2005)
• Challenge Protocol: Vaccination of heifers followed by intraconjunctival challenge with virulent B. abortus S2308 at mid-gestation; measurement of antibody responses by ELISA, particle concentration fluorescence assay, and card test; lymphoproliferation assays; cytokine gene expression analysis in bovine macrophages. (Wyckoff et al, 2005)
• Efficacy: SPEBA vaccine alone provided limited protection; co-administration with rBoIL 2 improved antibody responses and lymphoproliferation but protection against abortion was not as strong as with S19 plus rBoIL 2. (Wyckoff et al, 2005)
• Description: Vaccination of heifers followed by intraconjunctival challenge with virulent B. abortus S2308 at mid-gestation; measurement of antibody responses by ELISA, particle concentration fluorescence assay, and card test; lymphoproliferation assays; cytokine gene expression analysis in bovine macrophages. (Wyckoff et al, 2005)