• Vaccine Name: live attenuated Brucella ovis Delta Omp25 vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009148
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice)
• Antigen: Omp25 deletion (Deltaomp25)
• Preparation: Live attenuated Brucella ovis strain with omp25 gene disruption (BO25). (Edmonds et al., 2002)
• Description: Omp25 was examined as a potential vaccine candidate by creating a mutant strain with disruption of the omp25 gene. Efficacy: Vaccination with BO25 resulted in clearance of the challenge strain and provided 2.5 log10 greater protection against virulent B. ovis than vaccine strain Rev. 1. (Edmonds et al., 2002)

Mouse Response

• Host Strain: BALB/c
• Challenge Protocol: BALB/c mice were vaccinated with BO25 and challenged with virulent Brucella ovis; bacterial loads in spleens were measured. (Edmonds et al., 2002)
• Efficacy: Vaccination with BO25 resulted in clearance of the challenge strain and provided 2.5 log10 greater protection against virulent B. ovis than vaccine strain Rev. 1. (Edmonds et al., 2002)
• Description: BALB/c mice were vaccinated with BO25 and challenged with virulent Brucella ovis; bacterial loads in spleens were measured. (Edmonds et al., 2002)