• Vaccine Name: live attenuated Brucella melitensis delta Omp25 vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009147
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Goat
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice)
• Antigen: Omp25 deletion (Deltaomp25)
• Preparation: Live attenuated Brucella melitensis strain with omp25 gene disruption (BM25). (Edmonds et al., 2002)
• Description: Omp25 was examined as a potential vaccine candidate by creating a mutant strain with disruption of the omp25 gene. Efficacy: Vaccination with BM25 resulted in more than a 2 log10 reduction in bacterial load following challenge with virulent B. melitensis (P<0.01). (Edmonds et al., 2002)

Mouse Response

• Host Strain: BALB/c
• Challenge Protocol: BALB/c mice were vaccinated with BM25 and challenged with virulent Brucella melitensis; bacterial loads in spleens were measured. (Edmonds et al., 2002)
• Efficacy: Vaccination with BM25 resulted in more than a 2 log10 reduction in bacterial load following challenge with virulent B. melitensis (P<0.01). (Edmonds et al., 2002)
• Description: BALB/c mice were vaccinated with BM25 and challenged with virulent Brucella melitensis; bacterial loads in spleens were measured. (Edmonds et al., 2002)