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Vaccine Detail
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Brucella abortus strain RB51 HSP65 live virulent vaccine |
| Vaccine Information |
- Vaccine Name: Brucella abortus strain RB51 HSP65 live virulent vaccine
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009125
- Type: Live virulent vaccine
- Status: Research
- Host Species for Licensed Use: Cattle
- Host Species as Laboratory Animal Model: Mus musculus
- Antigen: 65-kDa heat shock protein (HSP65) of Mycobacterium bovis
- Preparation: Live attenuated strain RB51 bacteria transformed with plasmids expressing HSP65. (Vemulapalli et al, 2000a)
- Description: Recombinant strain RB51 constructed to express M. bovis HSP65 under Brucella groE promoter. Efficacy: Expression of HSP65 did not alter attenuation or vaccine efficacy of strain RB51 against B. abortus 2308 challenge. Immune response: Induced specific IgG2a antibodies and IFN-gamma secretion without IL-4, indicating a Th1 type immune response to HSP65. (Vemulapalli et al, 2000a)
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| Host Response |
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Mouse Response
- Immune Response: Induced specific IgG2a antibodies and IFN-gamma secretion without IL-4, indicating a Th1 type immune response to HSP65. (Vemulapalli et al, 2000a)
- Efficacy: Expression of HSP65 did not alter attenuation or vaccine efficacy of strain RB51 against B. abortus 2308 challenge. (Vemulapalli et al, 2000a)
- Description: Immunization of mice; measurement of specific antibody isotypes and cytokine secretion. (Vemulapalli et al, 2000a)
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| References |
Vemulapalli et al, 2000a: Vemulapalli R, He Y, Boyle SM, Sriranganathan N, Schurig GG. Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses. Infection and immunity. 2000 Jun; 68(6); 3290-6. [PubMed: 10816476].
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