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Vaccine Detail
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Brucella abortus strain RB51 beta-galactosidase live virulent vaccine |
| Vaccine Information |
- Vaccine Name: Brucella abortus strain RB51 beta-galactosidase live virulent vaccine
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009124
- Type: Live virulent vaccine
- Status: Research
- Host Species for Licensed Use: Cattle
- Host Species as Laboratory Animal Model: Mus musculus; macrophage-like J774A.1 cells
- Antigen: beta-galactosidase (lacZ)
- Preparation: Live attenuated strain RB51 bacteria transformed with plasmids expressing beta-galactosidase. (Vemulapalli et al, 2000a)
- Description: Recombinant strain RB51 constructed to express E. coli beta-galactosidase under Brucella groE or sodC promoters. Efficacy: Recombinant RB51 expressing beta-galactosidase maintained attenuation and protective efficacy against B. abortus 2308 challenge. Immune response: Induced specific IgG2a antibodies and IFN-gamma secretion without IL-4, indicating a Th1 type immune response to beta-galactosidase. (Vemulapalli et al, 2000a)
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| Host Response |
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Mouse Response
- Host cell type: J774A.1
- Immune Response: Induced specific IgG2a antibodies and IFN-gamma secretion without IL-4, indicating a Th1 type immune response to beta-galactosidase. (Vemulapalli et al, 2000a)
- Efficacy: Recombinant RB51 expressing beta-galactosidase maintained attenuation and protective efficacy against B. abortus 2308 challenge. (Vemulapalli et al, 2000a)
- Description: Enzyme assays for beta-galactosidase expression; heat shock and macrophage internalization; immunization of mice; antibody isotype and cytokine analysis. (Vemulapalli et al, 2000a)
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| References |
Vemulapalli et al, 2000a: Vemulapalli R, He Y, Boyle SM, Sriranganathan N, Schurig GG. Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses. Infection and immunity. 2000 Jun; 68(6); 3290-6. [PubMed: 10816476].
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