• Vaccine Name: live attenuated Brucella abortus strain 19 htrA gene deletion vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009109
• Type: Live attenuated vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Host Species as Laboratory Animal Model: Mus musculus (BALB/c mice)
• Antigen: htrA gene deletion mutant
• Preparation: Live attenuated Brucella abortus strain 19 with htrA gene deletion; kanamycin-resistant, ampicillin-sensitive. (Robertson et al, 1996)
• Description: An htrA deletion mutant was created in the bovine vaccine strain Brucella abortus S19 by replacing most of the htrA gene with a kanamycin resistance gene. Efficacy: The htrA mutant showed significantly reduced spleen colonization at 1 week and enhanced clearance at 4 and 7 weeks post-infection compared to parental strain; colonization was equivalent at 2 and 3 weeks. Immune response: The mutant elicited equivalent delayed type hypersensitivity and antibody responses compared to the parental strain in BALB/c mice. (Robertson et al, 1996)

Mouse Response

• Host Strain: BALB/c
• Immune Response: The mutant elicited equivalent delayed type hypersensitivity and antibody responses compared to the parental strain in BALB/c mice. (Robertson et al, 1996)
• Efficacy: The htrA mutant showed significantly reduced spleen colonization at 1 week and enhanced clearance at 4 and 7 weeks post-infection compared to parental strain; colonization was equivalent at 2 and 3 weeks. (Robertson et al, 1996)
• Description: In vitro susceptibility assays to H₂O2 and paraquat; in vivo spleen colonization and immune response studies in BALB/c mice. (Robertson et al, 1996)