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Vaccine Detail
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live attenuated Brucella abortus BCSP31 Salmonella chi 3781 vector vaccine |
| Vaccine Information |
- Vaccine Name: live attenuated Brucella abortus BCSP31 Salmonella chi 3781 vector vaccine
- Target Pathogen: Brucella spp.
- Target Disease: Brucellosis
- Vaccine Ontology ID: VO_0009101
- Type: Live attenuated vaccine + Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Pig
- Host Species as Laboratory Animal Model: Mus musculus; Sus scrofa
- Antigen: BCSP31 (31-kDa protein from Brucella abortus)
- Preparation: Live attenuated Salmonella choleraesuis chi 3781 mutant carrying recombinant plasmid pBA31-R7 expressing Brucella abortus BCSP31 protein. (Stabel et al, 1993)
- Description: An attenuated Salmonella choleraesuis strain chi 3781 mutant [delta cya-12 delta(crp-cdt)19] was engineered to express the 31-kDa protein (BCSP31) from Brucella abortus via plasmid pBA31-R7 for oral immunization. Efficacy: The recombinant Salmonella choleraesuis vaccine colonized gut mucosa and deep tissues in mice and swine; mice developed serum antibodies to BCSP31 and Salmonella endotoxin, while swine showed strong DTH responses to BCSP31 and Salmonella antigens but no significant antibody responses. Immune response: Mice developed serum antibodies to BCSP31 and Salmonella endotoxin and local intestinal antibody response to Salmonella endotoxin but not BCSP31; no DTH response to BCSP31 but positive to Salmonella antigen. Swine showed strong DTH responses to both BCSP31 and Salmonella soluble antigen, indicating cell-mediated immunity, confirmed by histology, but lacked significant antibody responses. (Stabel et al, 1993)
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| Host Response |
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| References |
Stabel et al, 1993: Stabel TJ, Mayfield JE, Morfitt DC, Wannemuehler MJ. Oral immunization of mice and swine with an attenuated Salmonella choleraesuis [delta cya-12 delta(crp-cdt)19] mutant containing a recombinant plasmid. Infection and immunity. 1993 Feb; 61(2); 610-8. [PubMed: 8423090].
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