• Vaccine Name: Brucella abortus strain 19 chemically modified salt-extractable protein (dCSP) subunit vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009069
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Cattle
• Antigen: chemically modified salt-extractable protein (dCSP)
• Adjuvant: Freund's complete adjuvant
• Preparation: dCSP formulated with Freund's complete adjuvant (Confer et al, 1987)
• Description: Beef heifers were vaccinated on Day 0 with chemically modified salt-extractable protein (dCSP) from Brucella abortus Strain 19 in Freund's complete adjuvant; a booster was given six weeks later with either homologous or heterologous vaccine. Efficacy: No significant protection observed; 60 to 83% of vaccinated heifers aborted and 70-83% were culture positive after challenge, similar to controls Immune response: Vaccination stimulated marked antibody responses and increased cell-mediated immune response measured by lymphocyte blast transformation; antibody responses increased rapidly after challenge and were higher in heifers that aborted; LBT responses were higher in vaccinates but not significantly different from controls (Confer et al, 1987)

Cattle Response

• Vaccination Protocol: Beef heifers were vaccinated on Day 0 with chemically modified salt-extractable protein (dCSP) from Brucella abortus Strain 19 in Freund's complete adjuvant; a booster was given six weeks later with either homologous or heterologous vaccine. (Confer et al, 1987)
• Immune Response: Vaccination stimulated marked antibody responses and increased cell-mediated immune response measured by lymphocyte blast transformation; antibody responses increased rapidly after challenge and were higher in heifers that aborted; LBT responses were higher in vaccinates but not significantly different from controls (Confer et al, 1987)
• Challenge Protocol: Vaccination of beef heifers followed by serologic tests, enzyme-linked immunosorbent assay, fluorometric immunoassay, lymphocyte blast transformation assay, and intraconjunctival challenge with Brucella abortus strain 2308. (Confer et al, 1987)
• Efficacy: No significant protection observed; 60 to 83% of vaccinated heifers aborted and 70-83% were culture positive after challenge, similar to controls (Confer et al, 1987)
• Description: Vaccination of beef heifers followed by serologic tests, enzyme-linked immunosorbent assay, fluorometric immunoassay, lymphocyte blast transformation assay, and intraconjunctival challenge with Brucella abortus strain 2308 (Confer et al, 1987)