• Vaccine Name: live attenuated Brucella abortus BCSP31 Salmonella chi 4064 vector vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009052
• Type: Live attenuated vaccine + Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Pig
• Host Species as Laboratory Animal Model: Mus musculus (BALB/cByJ mice)
• Antigen: 31-kDa protein (BCSP31) | BCSP31 (31-kilodalton protein)
• Preparation: Oral administration of S. typhimurium chi 4064 carrying plasmid pBA31-R7 expressing BCSP31 protein; no adjuvant used. (Stabel et al, 1991)
  Recombinant plasmid pBA31-R7 expressing BCSP31 was introduced into attenuated Salmonella typhimurium chi 4064 (delta cya delta crp mutant). (Stabel et al, 1990)
• Description: An attenuated Salmonella typhimurium chi 4064 mutant was used as a carrier for a recombinant plasmid pBA31-R7 coding for the Brucella abortus 31-kDa protein (BCSP31) to orally immunize swine. Efficacy: Orally immunized swine developed serum and intestinal antibody responses to BCSP31 and salmonella endotoxin; however, they did not develop delayed-type hypersensitivity or blastogenic responses to BCSP31. Parenteral immunization with BCSP31 plus Freund incomplete adjuvant did induce delayed-type hypersensitivity. Immune response: Effective stimulation of mucosal and systemic antibody-mediated immunity was observed after oral immunization with recombinant S. typhimurium expressing BCSP31, but no antigen-specific delayed-type hypersensitivity or blastogenic response was induced by this route. (Stabel et al, 1991)
  An attenuated Salmonella typhimurium chi 4064 mutant was used as a carrier for a plasmid coding for the Brucella abortus 31-kDa protein (BCSP31). Efficacy: The vaccine induced serum and intestinal antibody responses to BCSP31 and Salmonella endotoxin but did not induce delayed-type hypersensitivity or strong cell-mediated immunity. Immune response: Effective stimulation of antibody-mediated immunity; poor induction of cell-mediated immune response to BCSP31 in BALB/cByJ mice. (Stabel et al, 1990)

Mouse Response

• Vaccination Protocol: Oral immunization of BALB/cByJ mice with recombinant Salmonella typhimurium expressing BCSP31; Western blot for protein expression; ELISA for antibody responses; delayed-type hypersensitivity test; antigen-specific blastogenic assays. (Stabel et al, 1990)
• Immune Response: Effective stimulation of antibody-mediated immunity; poor induction of cell-mediated immune response to BCSP31 in BALB/cByJ mice. (Stabel et al, 1990)
• Efficacy: The vaccine induced serum and intestinal antibody responses to BCSP31 and Salmonella endotoxin but did not induce delayed-type hypersensitivity or strong cell-mediated immunity. (Stabel et al, 1990)
• Description: Oral immunization of BALB/cByJ mice with recombinant Salmonella typhimurium expressing BCSP31; Western blot for protein expression; ELISA for antibody responses; delayed-type hypersensitivity test; antigen-specific blastogenic assays. (Stabel et al, 1990)