• Vaccine Name: inactivated Brucella ovis saline-in-oil vaccine
• Target Pathogen: Brucella spp.
• Target Disease: Brucellosis
• Vaccine Ontology ID: VO_0009033
• Type: Inactivated vaccine
• Status: Research
• Host Species for Licensed Use: Sheep
• Host Species as Laboratory Animal Model: Ovis aries
• Adjuvant: oil | saline in-oil
• Preparation: Killed (inactivated) vaccine formulated with oil adjuvant. (Masalski, 1978)
  Inactivated Brucella ovis formulated as a saline-in-oil emulsion. (Bailey et al, 1988)
  Inactivated Brucella ovis vaccine formulated in saline in-oil adjuvant. (Bailey et al, 1987)
• Description: Two killed vaccines were produced using four strains of Brucella ovis, differing by the adjuvant used (hydroxide or oil). Vaccination was applied twice at an interval of 20 days to rams under experimental and field conditions. Efficacy: Rams treated with the oil adjuvant vaccine built up solid immunity. Immune response: Induction of antibodies detectable by serological tests; postvaccinal antibodies disappeared after three months. (Masalski, 1978)
  The vaccine was administered to adult ewes and rams by intraperitoneal and subcutaneous routes to study local tissue reactions. Immune response: Local granulomatous inflammation around oily vaccine droplets; diffuse peritonitis observed in most animals; extraperitoneal inflammatory response at injection site noted; peritonitis persisted in approximately 20% of animals six months after intraperitoneal vaccination. (Bailey et al, 1988)
  The study compared four vaccination methods using the inactivated Brucella ovis saline in-oil vaccine in rams. Efficacy: Two spaced doses given subcutaneously induced a more consistent, persistent, and higher magnitude serological response than a single dose or intraperitoneal administration. Immune response: The serological titre rose more rapidly and reached higher levels with two subcutaneous injections ten weeks apart; single intraperitoneal injection was least effective. (Bailey et al, 1987)

Sheep Response

• Immune Response: The serological titre rose more rapidly and reached higher levels with two subcutaneous injections ten weeks apart; single intraperitoneal injection was least effective. (Bailey et al, 1987)
• Efficacy: Two spaced doses given subcutaneously induced a more consistent, persistent, and higher magnitude serological response than a single dose or intraperitoneal administration. (Bailey et al, 1987)
• Description: Serological response of rams to one or two doses administered subcutaneously or intraperitoneally. (Bailey et al, 1987)

Sheep Response

• Vaccination Protocol: Adult ewes received inactivated Brucella ovis saline-in-oil vaccine by intraperitoneal and subcutaneous administration. (Bailey et al, 1988)
• Side Effects: Local granulomatous inflammation around oily vaccine droplets; diffuse peritonitis observed in most animals; extraperitoneal inflammatory response at injection site noted; peritonitis persisted in approximately 20% of animals six months after intraperitoneal vaccination. (Bailey et al, 1988)
• Description: Necropsy examination of vaccinated animals at intervals from 24 hours to six months post-injection to assess local inflammatory reactions and peritonitis. (Bailey et al, 1988)

Sheep Response

• Vaccination Protocol: Vaccination was applied twice at an interval of 20 days to rams under experimental and field conditions. (Masalski, 1978)
• Immune Response: Induction of antibodies detectable by serological tests; postvaccinal antibodies disappeared after three months. (Masalski, 1978)
• Challenge Protocol: Experimental vaccination of rams followed by challenge with virulent Brucella ovis strain on day 45 post-vaccination; serological tests (complement-fixation test, hemagglutination reaction, agar gel immunodiffusion), clinical and bacteriological examination of semen samples. (Masalski, 1978)
• Efficacy: Rams treated with the oil adjuvant vaccine built up solid immunity. (Masalski, 1978)
• Description: Experimental vaccination of rams followed by challenge with virulent Brucella ovis strain on day 45 post-vaccination; serological tests (complement-fixation test, hemagglutination reaction, agar gel immunodiffusion), clinical and bacteriological examination of semen samples. (Masalski, 1978)