• Vaccine Name: F. tularensis FopB Protein Vaccine
• Target Pathogen: Francisella tularensis
• Target Disease: Tularemia
• Vaccine Ontology ID: VO_0004034
• Type: Subunit vaccine
• Status: Research
• Antigen: .5 μg recombinant FopB protein
• FopB gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001237
  • Description: 10 μg of CpG oligodeoxynucleotide (5′-TCCATGACGTTCCTGACGTT-3′; Operon, Huntsville, AL)
• Immunization Route: intranasal immunization

Mouse Response

• Host Strain: C57BL/6 and HLA-DR4 tg
• Vaccination Protocol: mice were anesthetized i.n. with 3% isoflurane by use of a rodent anesthesia system and were immunized i.n. on day 0 with 7.5 μg rFopB in 25 μl of sterile PBS. This was accompanied by immunization with 10 μg of CpG oligodeoxynucleotide on days −1, 0, and +1. Mice were boosted once on day 14 with the same amount of antigen (Yu et al., 2010).
• Challenge Protocol: Mice were challenged i.n. with approximately 3.5 × 10^4 CFU (∼5 50% lethal doses [LD50s]) of LVS on day 30 (Yu et al., 2010).
• Efficacy: Intranasal vaccination of mice with rFopB conferred 50% protection (P < 0.05) against an otherwise lethal pulmonary challenge of 5 LD50s of LVS (3.5 × 10^4 CFU) in both C57BL/6 and HLA-DR4 tg mice compared with the protection conferred to LVS-challenged mock-vaccinated mice, all of which succumbed to the infection by day 10 (Yu et al., 2010).
• Host Ifng (Interferon gamma) response
  • Description: Spleens were collected at 14 days postvaccination, and single cells were prepared and assayed for FopB- and LVS-induced IFN-γ production. Differences in IFN-γ stimulation between rFopB- and mock-vaccinated mice were significant (Yu et al., 2010).
  • Detailed Gene Information: Click Here.