• Vaccine Name: pcDNA3-LiP0
• Target Pathogen: Leishmania infantum
• Target Disease: Infantile visceral leishmaniasis
• Product Name: DNA Vaccine encoding LIPO-A Protein of L. infantum
• Vaccine Ontology ID: VO_0004197
• Type: DNA vaccine
• Status: Research
• Antigen: LiP0
• LIPO-A gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid: pcDNA3 DNA vaccine plasmid
  • DNA vaccine plasmid name: pcDNA3 DNA vaccine plasmid
  • DNA vaccine plasmid VO ID: VO_0000132
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: In DNA immunization experiments, mice were inoculated twice intramuscularly (i.m.) in both quadriceps with 100 μg of DNA (50 μg per leg) of either pcDNA3-LiP0 or pcDNA3 (controls) in a total volume of 100 μl of PBS. When “prime-boost” immunization was carried out, two inoculations of DNA and two inoculations of recombinant protein were administered. In all groups, the mice were inoculated at 2-week intervals (Iborra et al., 2003).
• Challenge Protocol: Two weeks after the final inoculation, immunized mice were challenged with 5 × 10^4 stationary-phase promastigotes of L. major that were suspended in 50 μl of PBS and injected into the left hind footpad (Iborra et al., 2003).
• Efficacy: Three weeks after challenge, the parasite burden was found to be significantly lower in mice vaccinated with pcDNA3-LiP0 than in controls. Mice vaccinated with LiP0-DNA had a 99.1% reduction in the parasite burden 3 weeks after infection compared with mice vaccinated with control DNA (Iborra et al., 2003).