• Vaccine Name: S. agalactiae Rib Protein Vaccine
• Target Pathogen: Streptococcus agalactiae
• Target Disease: Group B streptococcus (GBS) infection
• Vaccine Ontology ID: VO_0004051
• Type: Subunit vaccine
• Status: Research
• Antigen: Purified Rib protein
• rib gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000139
  • Description: 0.1 ml of complete Freund’s adjuvant.
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: C3H/HeN
• Vaccination Protocol: Male C3H/HeN mice, 7 to 13 weeks old, were immunized subcutaneously with 25 μg of pure protein (Rib or bovine serum albumin [BSA]) in 0.1 ml of phosphate-buffered saline (PBS) mixed with 0.1 ml of complete Freund’s adjuvant. Four weeks later the mice were given boosters of the same amount of protein with incomplete Freund’s adjuvant (Larsson et al., 1996).
• Challenge Protocol: Two weeks after the booster, the mice were injected intraperitoneally (i.p.) with a 90% lethal dose (LD90) of log-phase bacteria, diluted in 0.5 ml of Todd-Hewitt broth. The LD90 for the different strains varied between 10^6 and 10^7 bacteria, as determined in preliminary experiments (Larsson et al., 1996).
• Efficacy: Vaccination of mice with the Rib protein protected against two strains of capsular type III and two strains of type II (Larsson et al., 1996).