• Vaccine Name: S. aureus DNA Vaccine encoding PBP2a Protein
• Target Pathogen: Staphylococcus aureus
• Target Disease: Staph infections
• Vaccine Ontology ID: VO_0004192
• Type: DNA vaccine
• Status: Research
• MecA gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000214
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Three days before the first immunization, bupivacain chloridrate 0.5% was injected at a dose of 2.5 μl/g of animal weight in the left quadriceps muscle. For DNA vaccination, three intramuscular injections with 10 μg of plasmid pCI-Neo-mecA or pCI-Neo only (negative control) diluted in 50 μl of sterile PBS were given at 2 weeks intervals into the left quadriceps muscle of mice (Senna et al., 2003).
• Challenge Protocol: To assess the immune response against MRSA, mice immunized with pCI-Neo and pCI-Neo-mecA were challenged with MRSA (MRSA HPS-03). Mice received a sublethal dose of 2.0×10^6 cfu intraperitonially daily for 7 days. A non-immunized group receiving the same bacterial dose was included as positive control.
• Efficacy: After challenge, the number of bacteria from kidneys from immunized and non-immunized mice was determined. Kidneys from immunized mice had 1000 times less on bacteria than the positive controls (non-immunized mice) (Senna et al., 2003).