• Vaccine Name: S. aureus FnbA Protein Vaccine
• Target Pathogen: Staphylococcus aureus
• Target Disease: Staph infections
• Vaccine Ontology ID: VO_0004049
• Type: Subunit vaccine
• Status: Research
• Antigen: Fibronectin binding domain of FnbA protein
• fnbA gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000139
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: BSVS
• Vaccination Protocol: Mice were vaccinated subcutaneously in the neck region with 20 μg of the antigens emulsified with complete Freund's adjuvant (primary vaccination) a few days after mating. Booster doses were given after 14 days with the same amount of immunogen, but emulsified with incomplete Freund's adjuvant (Mamo et al., 1994).
• Challenge Protocol: Mice were challenged 18-21 days after the booster dose by intramammary inoculation (mouse mastitis model) 23. Briefly, mice were anaesthetized using ether inhalation, the teat tips were aseptically removed and a 0.1 ml bacterial suspension (1 x l0^6 c.f.u, m1-1) was inoculated into the left quarter (L-4) and right quarter (R-4) mouse mammary glands (Mamo et al., 1994).
• Efficacy: Vaccination of mice with recombinant FnBP resulted in significant protection against challenge with S. aureus (Mamo et al., 1994).