• Vaccine Name: P. aeruginosa PcrV Protein Vaccine
• Target Pathogen: Pseudomonas aeruginosa
• Target Disease: Pseudomonas aeruginosa infection
• Vaccine Ontology ID: VO_0004191
• Type: Subunit vaccine
• Status: Research
• Antigen: PcrV
• PcrV gene engineering:
  • Type: Recombinant protein preparation
  • Description: PcrV was produced as a lipopolysaccharide-free histidine-tagged infusion protein in pET16b and was purified by nickel chromatography (Holder et al., 2001).
  • Detailed Gene Information: Click Here.
• Adjuvant: incomplete Freund's adjuvant
  • Adjuvant name: incomplete Freund's adjuvant
  • VO adjuvant ID: VO_0000142
  • Description: 0.1 ml of incomplete Freund's adjuvant (Holder et al., 2001).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: CF-1
• Vaccination Protocol: On day 0, groups of 10 female CF-1 mice weighing 22 to 25 g were immunized intramuscularly in the hind leg (10 μg of immunogen in 0.1 ml of incomplete Freund's adjuvant), followed by a booster dose (10 μg in saline) without adjuvant on day 14 (Holder et al., 2001).
• Challenge Protocol: Using the burned mouse model, mice were challenged by one of three strains of P. aeruginosa (Holder et al., 2001).
• Efficacy: Burned Pseudomonas aeruginosa-infected mice immunized against PcrV, a type III virulence system translocating protein, showed significantly enhanced survival compared to controls (Holder et al., 2001).