• Vaccine Name: P. aeruginosa OprI Protein Vaccine
• Target Pathogen: Pseudomonas aeruginosa
• Target Disease: Pseudomonas aeruginosa infection
• Vaccine Ontology ID: VO_0004190
• Type: Subunit vaccine
• Status: Research
• Antigen: Recombinant OprI protein
• OprI gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant: aluminum hydroxide vaccine adjuvant
  • Adjuvant name: aluminum hydroxide vaccine adjuvant
  • VO adjuvant ID: VO_0000127
  • Description: 100 μl of 1.5% Al(OH)3 (Finke et al., 1990).
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Female BALB/c mice, 12 to 16 weeks old, received 100 μl (27 ,ug) of OprI suspended in 100 μl of 1.5% Al(OH)3 intraperitoneally on days 0, 14, 35, and 63. Controls received Al(OH)3 only (Finke et al., 1990).
• Challenge Protocol: mice were challenged intraperitoneally 10 days after the last immunization with 200 μl of a P. aeruginosa serogroup 6 suspension. Mice vaccinated with recombinant OprI received 6 x 10^6 to 1 x 10^8 living organisms each. (Finke et al., 1990).
• Efficacy: Recombinant OprI protein was used to immunize mice, and was found to be protective against a challenge with a four- to fivefold 50% lethal dose of P. aeruginosa (Finke et al., 1990).