• Vaccine Name: B. burgdorferi DbpA and OspA Protein Vaccine
• Target Pathogen: Borrelia burgdorferi
• Target Disease: Lyme Disease
• Vaccine Ontology ID: VO_0004188
• Type: Subunit vaccine
• Status: Research
• Antigen: DbpAN40-OspAN40
• DbpA gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• OspA gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant: complete Freund's adjuvant
  • Adjuvant name: complete Freund's adjuvant
  • VO adjuvant ID: VO_0000139
• Adjuvant: incomplete Freund's adjuvant
  • Adjuvant name: incomplete Freund's adjuvant
  • VO adjuvant ID: VO_0000142
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: C3H/HeJ
• Vaccination Protocol: Mice were immunized by intraperitoneal injection of 10 μg of DbpAN40, 10 μg of OspAN40, 5 μg of DbpAN40 plus 5 μg of OspAN40, or 2.5 μg of E. coli protein extract with complete Freund's adjuvant and then, 4 weeks later, were given a second immunization of protein in incomplete Freund's adjuvant (Hanson et al., 2000).
• Challenge Protocol: At week 6, five of the mice in each immunization group were challenged by subcutaneous injection, into the dorsolateral thorax, of cloned B. burgdorferi N40 (Hanson et al., 2000).
• Efficacy: All mice immunized with the combined DbpAN40-OspAN40 vaccine (5 μg of each antigen) were protected against even the highest challenge dose of 10^6 spirochetes (Hanson et al., 2000).