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Vaccine Detail
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LC16m0 |
| Vaccine Information |
- Vaccine Name: LC16m0
- Target Pathogen: Variola virus
- Target Disease: Smallpox
- Vaccine Ontology ID: VO_0004098
- Type: Live attenuated
- Preparation: The Lister strain (Elstree) was one of the vaccinia viruses employed in the preparation of vaccines which were widely used in the World Health Organization smallpox eradication program with an acceptable safety record. The LC16 strains were first selected through 36 passages of the original Lister (LO) strain in primary rabbit kidney cells at 30°C. After a further 6 passages, LC16m0 was selected as a temperature-sensitive and medium pock-forming virus, and LC16m8 was cloned from the LC16m0 strain as a small pock-forming variant. The LC16m0 strain was shown to be satisfactorily attenuated in terms of fever response by inoculating nearly 1000 subjects, and the LC16m8 strain was confirmed to be more attenuated, especially in terms of dermal reaction, by tests on more than 100,000 children in Japan in 1974 and 1975, and was licensed as the first attenuated smallpox vaccine (Sugimoto et al., 1994).
- Virulence: Both LC16m8 and LC16m0 strains are temperature-sensitive and revealed much lower neurovirulence in cynomolgus monkeys and rabbits than the parental virus (LO): viral growth of LC16m0 and LC16m8 in the brain of rabbits and cynomolgus monkeys was at a lower level than that of LO. The genes responsible for neurovirulence and temperature sensitivity do not seem to correlate with the B5R gene and remain to be identified. The identification of these genes will make it possible to improve the VV vectors. The neurovirulence of RVVs tended to be milder than that of the parental vaccinia viruses. The neurovirulence of the WR strain and its recombinant was strongest, followed by that of LO1/LO-RVV (Sugimoto et al., 1994).
- Description: The LC16m0 strain is one of several temperature-sensitive and further attenuated variants derived from the Lister (Elstree) strain of vaccinia virus, which has a proven safety record in human populations. Several types of recombinant vaccinia viruses expressing a foreign antigen gene from a pathogenic virus have been constructed using the LC16m0 strain as a vector, and their immunological and virological characteristics have been investigated extensively (Sugimoto et al., 1994).
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| References |
Sugimoto et al., 1994: Sugimoto M, Yamanouchi K. Characteristics of an attenuated vaccinia virus strain, LC16m0, and its recombinant virus vaccines. Vaccine. 1994 Jun; 12(8); 675-81. [PubMed: 8091843].
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