• Vaccine Name: Cancer DNA Vaccine encoding TEM8
• Target Pathogen: Cancer
• Target Disease: Cancer
• Vaccine Ontology ID: VO_0011369
• Type: DNA vaccine
• Status: Research
• Immunization Route: Not specified

Mouse Response

• Host Strain: C57BL/6J
• Vaccination Protocol: After tumor challenge, mice were vaccinated with PBS, pOVA, or pTEM8 once a week for 3 times (Ruan et al., 2009).
• Challenge Protocol: To explore whether the DNA vaccine has antitumor function in vivo, 3 days before the first immunization, C57BL/6J mice were challenged subcutaneous injection of 1×10^4 B16F10 cells into the left flank to induce primary tumor model or intravenous injection with 1×10^5 B16F10 cells to induce experimental pulmonary metastases model (Ruan et al., 2009).
• Efficacy: The tumor volume expanded rapidly after 25 days of tumor challenge in the PBS and pOVA groups. However, in the pTEM8 group, the tumor volume expanded steadily. The death occurrence only happened after 40 days of tumor challenge in the pTEM8 group. Moreover, the mean lifespan of mice in the pTEM8 group was prolonged remarkably. In addition, a marked reduction in dissemination of experimental pulmonary metastases was observed in the pTEM8 group compared with control groups. These data demonstrated that the pTEM8 had superior antitumor efficiency (Ruan et al., 2009).