• Vaccine Name: Rickettsia DNA Vaccine encoding OmpB Protein boosted with Recombinant OmpB
• Target Pathogen: Rickettsia spp
• Target Disease: Spotted Fever, Typhus
• Vaccine Ontology ID: VO_0011389
• Type: DNA vaccine
• Status: Research
• ompB gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005062
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: C3H/ HeN
• Vaccination Protocol: Animals were immunized with only two doses of DNA (100 μg of recombinant plasmid and 100 μg of pIL-12) four weeks apart given intramuscularly in the tibialis anterior muscles. All mice that received DNA immunizations were also administered two booster immunizations of 100 μg each of the homologous purified recombinant protein. Control mice received either non-recombinant vector plus β-galactosidase-His recombinant protein (negative control) or a sublethal immunizing dose of R. conorii (positive
  control) (Díaz-Montero et al., 2001).
• Challenge Protocol: Mice were challenged with 3 median lethal doses (LD50) of R. conorii. In a previous experiment, this dose was shown to kill 100% of naive mice. After challenge, animals were observed daily for morbidity and mortality (Díaz-Montero et al., 2001).
• Efficacy: R. rickettsii OmpB contain B and T lymphocyte epitopes. Immunizations with each of two fragments from OmpB (rompB1550-2738 and rompB2459-4123) conferred protection against challenge with a lethal dose of R. conorii. Protection appeared to be better achieved among the groups that received both DNA and recombinant protein immunizations, although recombinant protein immunizations alone provided some protection (Díaz-Montero et al., 2001).