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Vaccine Detail

Influenza virus DNA vaccine encoding HA from Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1))
Vaccine Information
  • Vaccine Name: Influenza virus DNA vaccine encoding HA from Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1))
  • Target Pathogen: Influenza virus
  • Target Disease: Influenza (flu)
  • Vaccine Ontology ID: VO_0004020
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) HA hemagglutinin
  • HA from Influenza A virus (A/Thailand/1(KAN-1)/2004(H5N1)) gene engineering:
    • Type: DNA vaccine construction
    • Description: Plasmid encoding HA (A/Thailand/1(KAN-1)/2004, GenBank AY555150) was synthesized using human-preferred codons and constructed in a CMV/R backbone by GeneArt (Regensburg, Germany) (Rao et al., 2010).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid:
    • DNA vaccine plasmid name:
    • DNA vaccine plasmid VO ID: VO_0005004
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: BALB/c mice (10 animals for each group of HA alone, HA+NP, and HA+NP+M2; and 5 animals for the NP alone and vector control) were immunized three times with a total of 15 µg plasmid DNA in 100 µl of PBS (pH 7.4) intramuscularly at 0, 3, and 6 weeks. For the single HA or NP plasmid group, each group received 5 µg DNA for each plasmid with 10 µg of control vector as filler DNA (total 15 µg) (Rao et al., 2010).
  • Challenge Protocol: Viral challenge experiments were performed 3 weeks after the last immunization. All challenged animals were exposed under CO2 anesthesia to an intranasal viral inoculum of 100 LD50 of A/Vietnam/1203/2004 virus. Body weight and survival were monitored for 21 days (Rao et al., 2010).
  • Efficacy: All animals in the control and NP alone groups died within 6 days after viral challenge, whereas animals immunized with HA alone, HA+NP and HA+NP+M2 showed survival rates of 100, 90 and 70%, respectively (Rao et al., 2010).
References
Rao et al., 2010: Rao SS, Kong WP, Wei CJ, Van Hoeven N, Gorres JP, Nason M, Andersen H, Tumpey TM, Nabel GJ. Comparative efficacy of hemagglutinin, nucleoprotein, and matrix 2 protein gene-based vaccination against H5N1 influenza in mouse and ferret. PloS one. 2010; 5(3); e9812. [PubMed: 20352112].