• Vaccine Name: Flagellin-based HA globular head fusion vaccine using HA protein from Influenza A virus (A/Viet Nam/1203/2004(H5N1))
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004175
• Type: Subunit vaccine
• Status: Research
• Antigen: Influenza A virus (A/Viet Nam/1203/2004(H5N1)) HA hemagglutinin
• HA from Influenza A virus (A/Viet Nam/1203/2004(H5N1)) gene engineering:
  • Type: Recombinant protein preparation
  • Description: Panel of flagellin-based hemagglutinin (HA) globular head fusion vaccines in inbred mice. The HA globular head of these vaccines was derived from the A/Vietnam/1203/04 (VN04; H5N1) HA molecule (Song et al., 2009).
  • Detailed Gene Information: Click Here.
• Immunization Route: Subcutaneous injection

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Six-week-old female BALB/c mice (Harlan) were vaccinated subcutaneously (s.c.) with two or three doses of vaccine (day −28, −14) or (−42, −28, −14) in 100 μl of vehicle (F147). The animals were bled 7 or 12 days following the last vaccination. Seroconversion was then evaluated via HAI assay. For efficacy studies, H5N1 infection was subsequently performed (Song et al., 2009).
• Challenge Protocol: Prior to virus infection, anesthesia was performed using 5% isofluorane. The mice were then infected intranasally (i.n.) with influenza A/Vietnam/1203/04 at a dose determined in units of TCID50 per animal of H5N1 in 40 μl of PBS (day 0) (Song et al., 2009).
• Efficacy: Researchers evaluated the immunogenicity and efficacy of a panel of flagellin-based hemagglutinin (HA) globular head fusion vaccines in inbred mice. The HA globular head of these vaccines was derived from the A/Vietnam/1203/04 (VN04; H5N1) HA molecule. Results show that replacement of domain D3 of flagellin with the VN04 HA globular head creates a highly effective vaccine that elicits protective HAI titers which protect mice against disease and death in a lethal challenge model with H5 VN virus (Song et al., 2009).