• Vaccine Name: pCAGGoptiHA
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004173
• Type: DNA vaccine
• Status: Research
• Antigen: Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) HA hemagglutinin
• HA from Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) gene engineering:
  • Type: DNA vaccine construction
  • Description: Both the wild-type HA gene of GS/GD/96 virus and optiHA were cloned into the plasmid vector pCAGGS (kindly provided by Dr. Y. Kawaoka) under the control of the chicken β-actin promoter, and plasmid pCI (Promega) under the control of the cytomegalovirus (CMV) promoter (Jiang et al., 2007).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000099
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Vaccination Protocol: The plasmids were diluted to the desired concentration and were administered by intramuscular injection in a 200 μl volume at the leg muscle (Jiang et al., 2007).
• Challenge Protocol: Chickens were challenged with 1000 LD50 of H5N1 viruses in 0.1 ml intranasally. Oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in eggs, and chickens were observed daily for disease signs and death for 2 weeks (Jiang et al., 2007).
• Efficacy: Results indicated that two doses of 10 microg of pCAGGoptiHA derived from Influenza A virus (A/Goose/Guangdong/1/96(H5N1)) could induce complete protection in chickens against H5 lethal virus challenge (Jiang et al., 2007).