• Vaccine Name: L. major DNA Vaccine encoding SP
• Target Pathogen: Leishmania major
• Target Disease: Cutaneous leishmaniasis
• Vaccine Ontology ID: VO_0004172
• Type: DNA vaccine
• Status: Research
• SP gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • DNA vaccine plasmid name: pcDNA3.1 DNA vaccine plasmid
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Subcutanteous injection

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Immunization experiments were carried out twice at 3 weeks intervals in five groups of 10 BALB/c mice. Mice in group I (DNA/DNA) were immunized subcutaneously (s.c.) in the right footpad with 100 μg of pcDNA-sp plasmid, in the both prime and boost vaccination. Two control groups (IV and V) were studied; mice in group IV (vector only/CpG) were inoculated using 100 μg of pcDNA (vector without insert) in the first vaccination and the same adjuvant regimen (50 μg CpG ODN and 70 (v/v) Montanide 720) in the second vaccination. Mice in group V (PBS) received PBS alone (Rafati et al., 2006).
• Challenge Protocol: Animals were then challenged 3 weeks after completion of the immunization protocol with 3 × 10^5 L. major MRHO/IR/75/ER metacyclic promastigotes that had been suspended in 50 μl PBS and injected into the left footpad (Rafati et al., 2006).
• Efficacy: DNA/DNA strategy developed more effective protective responses and induced 81% reduction in L. major parasite load (Rafati et al., 2006).