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Vaccine Detail

Hepatitis B virus DNA vaccine encoding HBVgp2 pre-S1/pre-S2/S
Vaccine Information
  • Vaccine Ontology ID: VO_0011411
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Hepatitis B virus HBVgp2 pre-S1/pre-S2/S
  • S gene engineering:
    • Type: DNA vaccine construction
    • Description: The pCI expression vector (Promega, Charbonières, France) was used to clone the entire DHBV large envelope gene into Not I polylinker site leading to the pCI-preS/S plasmid [5] and the DHBV core gene leading to pCI-C plasmid (Thermet, submitted). The pCI-preS/S, pCI-C and the native pCI plasmids were purified by Endotoxin Free Giga prep (Qiagen, Hilden, Germany) (Thermet et al., 2003).
    • Detailed Gene Information: Click Here.
  • Vector: pCI expression vector (Promega, Charbonières, France)
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Ducks Response

  • Host Strain: Pekin
  • Vaccination Protocol: Four-week-old ducks received intramuscular (i.m.) injections of 100–300 μg of plasmid DNA diluted in NaCl 0.9%. The birds were injected in three sites (anterior quadriceps of both legs and breast), and booster doses were given 3 weeks later at the same sites (Thermet et al., 2003).
  • Challenge Protocol: Progeny ducklings received a high titre DHBV challenge and the viremia was followed by quantitative dot blot hybridisation for each animal during 17 days (Thermet et al., 2003).
  • Efficacy: Immunisation with a plasmid encoding the DHBV large (L) envelope protein (HBVgp2 pre-S1/pre-S2/S) induced a strong, specific, highly neutralising and long-lasting anti-preS humoral response in uninfected ducks. Importantly, maternal antibodies elicited by such DNA immunisation were vertically transmitted and protected progeny against viral challenge (Thermet et al., 2003).
References
Thermet et al., 2003: Thermet A, Rollier C, Zoulim F, Trepo C, Cova L. Progress in DNA vaccine for prophylaxis and therapy of hepatitis B. Vaccine. 2003; 21(7-8); 659-662. [PubMed: 12531335].