• Vaccine Ontology ID: VO_0011469
• Type: DNA vaccine
• Status: Research
• Antigen: Eimeria tenella antigen gene (3-1E) and chicken interferon gamma gene (ChIFN-gamma)
• 3-1E gene engineering:
  • Type: DNA vaccine construction
  • Description: The Eimeria tenella antigen gene (3-1E) and chicken interferon gamma gene (ChIFN-gamma) were subcloned into the mammalian expression vector proVAX forming the plasmids proE and prol, and then linked by splicing overlap extension by polymerase chain reaction to construct the chimeric plasmid prolE; the chimeric protein (rlE) was expressed in Escherichia coli harboring the constructed plasmid pGEX/IE (Xu et al., 2006).
  • Detailed Gene Information: Click Here.
• Vector: proVAX
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Host Strain: Broiler
• Vaccination Protocol: Broilers were administered two intramuscular injections with the constructed DNA vaccines (50 microg); in the protein booster groups 100 microg of the rlE were given following the proIE prime (Xu et al., 2006).
• Challenge Protocol: One week following the booster immunization, all chickens except for the unchallenged control group were orally challenged with 5 x 10^4 sporulated E. tenella GS oocysts (Xu et al., 2006).
• Efficacy: The Eimeria tenella antigen gene (3-1E) and chicken interferon gamma gene (ChIFN-gamma) were subcloned into the mammalian expression vector proVAX forming the plasmids proE and prol. After challenge the proIE-vaccinated chickens showed protective immunity as demonstrated by significantly reduced oocyst shedding (Xu et al., 2006).