• Vaccine Ontology ID: VO_0004533
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Chickens
• VP2 gene engineering:
  • Type: DNA vaccine construction
  • Description: The synthetic gene for VP2 protein of vvIBDV Gx with codons optimized for chicken usage was synthesized and cloned into pCAGGS vector (Gao et al., 2013).
  • Detailed Gene Information: Click Here.
• Vector: pCAGGS prime, rVP2 protein boost (Gao et al., 2013)
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Immune Response: Chickens in the DNA prime–protein boost group developed significantly higher levels of ELISA and neutralizing antibodies to IBDV compared with those immunized with either the DNA vaccine or the protein vaccine alone (P < 0.05). Furthermore, the highest levels of lymphocyte proliferation response, IL-4 and IFN-γ production were induced following priming with the DNA vaccine and boosting with the rVP2 protein (Gao et al., 2013).
• Efficacy: Chickens inoculated with the DNA prime–protein boost vaccine had 100% protection against challenge with vvIBDV, as evidenced by the absence of clinical signs, mortality, and bursal atrophy. In contrast, chickens receiving the DNA vaccine and the rVP2 protein vaccine had 67% and 80% protection, respectively (Gao et al., 2013).