• Vaccine Ontology ID: VO_0004525
• Type: DNA vaccine
• Status: Research
• Fusion protein gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: pCMV-F (Kapczynski and Sellers, 2003)
• Immunization Route: Intramuscular injection (i.m.)

Turkey Response

• Vaccination Protocol: One-week-old SPF poults were arbitrarily separated into four groups of five or six birds. All vaccinations were delivered by syringe injection into the thigh muscle in 0.1-ml aliquots. Control birds received PBS at days 7 and 21 of age. Birds receiving either pCMV, pCMV-F, or pCMV-N received 50 ag of plasmid DNA on day 7 and 100 ag on day 21 (Kapczynski and Sellers, 2003).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: Serum neutralizing antibodies were significantly higher in birds receiving pCMV-F compared with all other groups (Kapczynski and Sellers, 2003).
• Challenge Protocol: Two weeks after the second vaccination, birds were challenged via intranasal route with 1 x 10^5 TCID50 Mn la per bird (Kapczynski and Sellers, 2003).
• Efficacy: Poults receiving pCMV-F had significantly decreased levels of virus in tracheal swabs on all days in Experiment I and on day 7 in Experiment II compared with other groups. In addition, the percentage of birds displaying clinical signs of disease was decreased in turkeys receiving pCMV-F in both experiments. In experiments I and II, only 20% and 30%, respectively, of birds vaccinated with pCMV-F displayed clinical signs at day 7 (Kapczynski and Sellers, 2003).