• Vaccine Ontology ID: VO_0004361
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Macaque
• VP1 gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pIRES expressed major structural protein (capsid protein VP1) VP1 of coxsackievirus B3 (CVB3) (Henke et al., 2004).
  • Detailed Gene Information: Click Here.
• Vector: pIRES (Henke et al., 2004)
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: DNA immunizations with the major structural protein VP1 of coxsackievirus B3 (CVB3) have been previously found to protect mice from a lethal challenge with CVB3. Co-expression of the immune-stimulatory interleukin-2 (IL-2) can increase the efficacy of the inoculated DNA vaccine depending on the route of administration and the mouse strain used. After i.m. administration, IL-2 co-expression increased the protection rate by 18.3% whereby the g.g. inoculation was much less effective in BALB/c mice. In contrast, after g.g. inoculation IL-2 co-expression increased the protection rate by 36.7% whereby the i.m. was much less effective in C57BL/6 mice (Henke et al., 2004).