|
|
Eimeria maxima |
| Table of Contents |
- General Information
- NCBI Taxonomy ID
- Disease
- Introduction
- Vaccine Related Pathogen Genes
- gam56
(Protective antigen)
- gam56
(Protective antigen)
- gam82
(Protective antigen, Protective antigen)
- gam82
(Protective antigen)
- mic2
(Protective antigen)
- mic7
(Protective antigen)
- TFP250
(Protective antigen)
- Vaccine Related Host Genes
- IL15
- IL2
- Vaccine Information
- CoxAbic
- E. maxima DNA vaccine pcDNA-Gam56
- E. maxima Gam82 protein vaccine
- E. maxima oocysts with Arlacel A vaccine
- References
|
| I. General Information |
| 1. NCBI Taxonomy ID: |
|
5804 |
|
2. Disease: |
| Coccidiosis |
|
3. Introduction |
| Eimeria spp. are obligate intracellular apicomplexan protozoan parasites and are the cause of intestinal coccidiosis. Infections of the vertebrate host with these parasites result in the induction of potent protective immune responses, and protection against challenge infections is confined absolutely to homologous species, even though both T- and B-cell responses exhibit cross-species reactivity. Eimeria maxima is the most immunogenic of the seven species of Eimeria that infect the domestic chicken (Smith et al., 2002). |
| II. Vaccine Related Pathogen Genes |
|
1. gam56 |
-
Gene Name :
gam56
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
NCBI Gene ID :
25336657
-
NCBI Nucleotide GI :
AY129951.2
-
NCBI Protein GI :
25989587
-
Taxonomy ID :
5804
-
Gene Strand (Orientation) :
?
-
Protein Name :
56 kDa gametocyte antigen
-
Protein pI :
4.61
-
Protein Weight :
51819.73
-
Protein Length :
539
-
Protein Note :
tyrosine and serine rich gametocyte protein; involved in oocyst wall formation
-
Protein Sequence : Show Sequence
>AAN05087.1 56 kDa gametocyte antigen [Eimeria maxima]
MTRLGLAAVALALAVGPSMAVPSTTPVENQVHPYSEMSTYQEGSAPGAPEDTTTTTTSSPVSDGAEQWLE
SFVRAVQRQLQLQDQMMRQLMRDIQEYLSTAFNWAENQSTAYTRVTEMMDMISNRMNAAMDSSNELMTTS
DTTDPETLRRATRKYMKEVRVQDVLVDALWASLRGVQTAAWMNGVTAIEKEETTPMASRAAEEFLHRMYH
NLRAAGMSEEDVAKFIPRAEYNPSEQSRNMGRKGRSFYYGGYPSYYNSPYYSYSSYPSYYNYSYPSYSYS
SYPSYYRYSSYPYYNYSYPSYYNYGSYPYYSYSSYPSWYWRRLRSLATATCPDCPPLTTPSMIPTPPPMM
NMMNTPPPMANMMTSMMMNTPMVPPPRTLGTEAMSLGLAPIGITGAPMTGFGVPPEFGPFGAEGIGLPTD
ALGSTPEMTPFDPTTPYRTLAPMDLPPIPPPVFPETPMRPPTPFGFGPAPVPPMPF
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Xu et al., 2013)
- Related Vaccine(s):
E. maxima DNA vaccine pcDNA-Gam56
|
|
2. gam56 |
-
Gene Name :
gam56
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
NCBI Protein GI :
AAN05087
-
Taxonomy ID :
5804
-
Protein Name :
56 kDa gametocyte antigen
-
Protein pI :
4.61
-
Protein Weight :
51819.73
-
Protein Length :
539
-
Protein Note :
tyrosine and serine rich gametocyte protein; involved in oocyst wall formation
-
Protein Sequence : Show Sequence
>AAN05087.1 56 kDa gametocyte antigen [Eimeria maxima]
MTRLGLAAVALALAVGPSMAVPSTTPVENQVHPYSEMSTYQEGSAPGAPEDTTTTTTSSPVSDGAEQWLE
SFVRAVQRQLQLQDQMMRQLMRDIQEYLSTAFNWAENQSTAYTRVTEMMDMISNRMNAAMDSSNELMTTS
DTTDPETLRRATRKYMKEVRVQDVLVDALWASLRGVQTAAWMNGVTAIEKEETTPMASRAAEEFLHRMYH
NLRAAGMSEEDVAKFIPRAEYNPSEQSRNMGRKGRSFYYGGYPSYYNSPYYSYSSYPSYYNYSYPSYSYS
SYPSYYRYSSYPYYNYSYPSYYNYGSYPYYSYSSYPSWYWRRLRSLATATCPDCPPLTTPSMIPTPPPMM
NMMNTPPPMANMMTSMMMNTPMVPPPRTLGTEAMSLGLAPIGITGAPMTGFGVPPEFGPFGAEGIGLPTD
ALGSTPEMTPFDPTTPYRTLAPMDLPPIPPPVFPETPMRPPTPFGFGPAPVPPMPF
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Xu et al., 2013)
|
|
3. gam82 |
-
Gene Name :
gam82
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
VO ID :
VO_0011062
-
NCBI Gene ID :
25336658
-
NCBI Protein GI :
38565038
-
Taxonomy ID :
5804
-
Gene Strand (Orientation) :
?
-
Protein Name :
82 kDa gametocyte antigen
-
Protein pI :
4.8
-
Protein Weight :
63301.57
-
Protein Length :
661
-
Protein Note :
tyrosine and serine rich gametocyte precursor protein; involved in oocyst wall formation
-
Protein Sequence : Show Sequence
>AAO47083.2 82 kDa gametocyte antigen [Eimeria maxima]
MTRAAALAGVLALAAAGSSLALPTVLDTTTGTQVEWTETPLDTTEVTMGEMGSTTSGTTPTSTGVRMMEA
ETTTPSTPEAPQQQQQMPQPQPQPQQTTPVPEAVLEAIMQEMQNIFRSSLVPGWDTVGTAADAVRQIVTR
VRERLTGPLMMTEMDTGLGRTGPLSTTGATGATTGPVAALRGVTNDFLREIMIQEAVLETLWAVVRDAQE
RPWLVNEQEALHAATADAVQGFLGRMHDRLRATGFSEEEVMRLLPRSRNGGCTRTGGLFDQCNDAPPSRL
LGKRMYSTGYYGYGYPSYYSYGYSYPAYSHYPVSYPYYGYSWGPSYYYGSGYYGKHGYKYGHYYRRLAEQ
EPRPVMPPAAATAAANLRAAAAAAAEVPPPPPPAAVPPPPPAAAAGTPAMMPPPMMGVEEPVPFRSLYPS
YSWSYPAYTRVSPSYSYYTPSYSSSYYYPRYNYAYNYPLYSDYSWYDYSYPLAYSSYSSYPLSYSSYSYP
LSYTYPSAFYRRLEVPDLTTTTTTHHEQQQQQQQESTTTAVPTETITTPSTRNTHSSSLRRVGERYEPIT
PTQRTFYNNTEGTNNPVYTPENLTEDEPQTVWETYN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
Animals vaccinated with Gam82 and challenged with E. maxima showed lower oocyst shedding and reduced intestinal pathology compared with non-vaccinated and parasite-challenged animals. Gam82 vaccination also stimulated the production of antigen-specific serum antibodies and induced greater levels of IL-2 and IL-15 mRNAs compared with non-vaccinated controls. These results demonstrate that the Gam82 recombinant protein protects against E. maxima and augments humoral and cell-mediated immunity (Jang et al., 2010).
-
Additional Molecule Role :
Protective antigen
-
Additional Molecule Role Annotation :
(Jang et al., 2010)
- Related Vaccine(s):
E. maxima Gam82 protein vaccine
|
|
4. gam82 |
-
Gene Name :
gam82
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
NCBI Protein GI :
AAO47083
-
Taxonomy ID :
5804
-
Protein Name :
82 kDa gametocyte antigen
-
Protein pI :
4.8
-
Protein Weight :
63301.57
-
Protein Length :
661
-
Protein Note :
tyrosine and serine rich gametocyte precursor protein; involved in oocyst wall formation
-
Protein Sequence : Show Sequence
>AAO47083.2 82 kDa gametocyte antigen [Eimeria maxima]
MTRAAALAGVLALAAAGSSLALPTVLDTTTGTQVEWTETPLDTTEVTMGEMGSTTSGTTPTSTGVRMMEA
ETTTPSTPEAPQQQQQMPQPQPQPQQTTPVPEAVLEAIMQEMQNIFRSSLVPGWDTVGTAADAVRQIVTR
VRERLTGPLMMTEMDTGLGRTGPLSTTGATGATTGPVAALRGVTNDFLREIMIQEAVLETLWAVVRDAQE
RPWLVNEQEALHAATADAVQGFLGRMHDRLRATGFSEEEVMRLLPRSRNGGCTRTGGLFDQCNDAPPSRL
LGKRMYSTGYYGYGYPSYYSYGYSYPAYSHYPVSYPYYGYSWGPSYYYGSGYYGKHGYKYGHYYRRLAEQ
EPRPVMPPAAATAAANLRAAAAAAAEVPPPPPPAAVPPPPPAAAAGTPAMMPPPMMGVEEPVPFRSLYPS
YSWSYPAYTRVSPSYSYYTPSYSSSYYYPRYNYAYNYPLYSDYSWYDYSYPLAYSSYSSYPLSYSSYSYP
LSYTYPSAFYRRLEVPDLTTTTTTHHEQQQQQQQESTTTAVPTETITTPSTRNTHSSSLRRVGERYEPIT
PTQRTFYNNTEGTNNPVYTPENLTEDEPQTVWETYN
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Jang et al., 2010)
|
|
5. mic2 |
-
Gene Name :
mic2
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
NCBI Gene ID :
25336734
-
NCBI Nucleotide GI :
FR718971.1
-
NCBI Protein GI :
CBX60033
-
Other Database IDs :
CDD:115333
InterPro: IPR009556
UniProtKB/TrEMBL: F6IA05
-
Taxonomy ID :
5804
-
Protein Name :
Microneme protein 2
-
Protein pI :
4.65
-
Protein Weight :
28403.732
-
Protein Length :
350
-
Protein Note :
Microneme protein Etmic-2; pfam06670
-
DNA Sequence : Show Sequence
>FR718971.1 Eimeria maxima mic2 gene for Microneme protein 2, exons 1-4, strain Houghton, clone H.cl-2006:1
CAGCACAATGGCTCGCGCCCTTTCATTGATCGCCCTGGGTCTGCTTTTTTCCCTGCCTTCAACTTCGGCG
GTCAGGACCAAAGTACCAGGTTAGCAAATGCTAATGAAATTCACGAATTTGGGGGCTCTATGGGAGGCAG
TGTTTCATGAGGTCACTGTGCAGAGGAGCAGTGAATGCACTGCAGAATAGTGTTTAAGGAACGTCCCTGC
ATTCTCACTTATAAAGGTGCTTAGAGCGTTTCTGATGGTGAAGAAAGAATGGGGACGCTTACCCAAAGGC
CTACTGTGGGGTGGATGCTTTCGATGAATGGCGGATTCCGGTGATGCCGTGCAAACATTGGCTGGGCTAC
AAGGCTTCAAGCATAGTAATGGTTATCGTGAGCTGCGAATGTGTGTTGAGCCTGGCGATTGTTAGAACTG
TAATGCGCTATGTATTGTGTTCAATGTGTACTTGCGCAGGGGATGAGCCTTCCGGTCCGGATTCTGCCCT
TACCGCCGGTAGTCCGACTAGGGGCAACCACGGTGCGTAATTTTGTCCTCCGTTTGACCCGGATTTGCGA
CACCGTTGACAGGTGGCACTTGCATCGGAATGTTGCATCAAATTATGTACGTATTTGCTTCCTCCTCCTT
TCAGGTGTAGGCGGATTTGCTGAAGCACATTGCAATAGACTAACTGTTCGCGGAGGTCTTCAAGAGAAGG
AAGCAGTGAAAGTTACTGCCAATGGATGGGGGAAAGGTGACGCAGACTTCTTTGTTGAGCTGGTTACTGA
TAATACTCGGGGCATGCTTCAGATAAGAGAATCACAGACTGAAGCGGGACCAGGGTTGGCTGGAGGGGGC
GGCCAGGAAGGGGGTAGTACCGAAGGGAGTAAAACTGATGAAGGGCCCGTTAAGGAACAGCCATCCATTC
CCATTGTTGGTGTCCGCATTCCTGGTTCCGCAAATGAAAATGGGGAATCACGTAAACCGGCCGTGTTGGT
TTACGGGGAAGGTGAGTCTGCTCCGAAAGAATTTCCTTTGGATTCTCCAGCTGGGCCGACATCACCTTTC
ATGGTCGTCCTTCAACAGAAATCGCCAACAGAAATGACTGTGCGGCTTTTTACATGGATACCAAAGTGAG
TTGGCACATTGGGGAACCGACTGGCCCTTGTATAGCTGGACCATCATGCTAGTCTTGGCGATTTGTGTGA
TCTAGTGGTTTGCTGTTACTTCCCCGGCCTCGGCATTTCTCGTTGGTGTATGGGGTGCATACGGTCTGTG
CATATTTAAATGTATGTGCAGCGGAAGTGGTGGAGACGGTTCATGGCACGAGACGTTTGTAGATGTTGGC
GTTGGGATCAACCATCGCGACGTTATGGTCGTTGTGAGCGACTGCGTCCCGCATAGCCTTAGAATTTACG
GTTCATCATCTGCAGATTTAGTGACGGCCGACGAGAAGACCTGTCAGGCAACTGAGCCGCAGTTAGTGAA
TCTGACTTCACCTCCCGAAAACAGAACCCATCCAGGTGCCGAGACATCAACCACAACATCGGTCAGCTCC
TAGAGAGGTAGAGCTTGACAAGACCAAAGGAGAGATGCCA
-
Protein Sequence : Show Sequence
>CBX60033.1 Microneme protein 2 [Eimeria maxima]
MARALSLIALGLLFSLPSTSAVRTKVPGDEPSGPDSALTAGSPTRGNHGVGGFAEAHCNRLTVRGGLQEK
EAVKVTANGWGKGDADFFVELVTDNTRGMLQIRESQTEAGPGLAGGGGQEGGSTEGSKTDEGPVKEQPSI
PIVGVRIPGSANENGESRKPAVLVYGEGESAPKEFPLDSPAGPTSPFMVVLQQKSPTEMTVRLFTWIPNG
SGGDGSWHETFVDVGVGINHRDVMVVVSDCVPHSLRIYGSSSADLVTADEKTCQATEPQLVNLTSPPENR
THPGAETSTTTSVSS
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Huang et al., 2015)
|
|
6. mic7 |
-
Gene Name :
mic7
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
NCBI Protein GI :
CBX60037
-
Other Database IDs :
UniProtKB/TrEMBL: G0LEU8
-
Taxonomy ID :
5804
-
Protein Name :
Microneme protein 7
-
Protein pI :
4.27
-
Protein Weight :
16737.882
-
Protein Length :
225
-
Protein Sequence : Show Sequence
>CBX60037.1 Microneme protein 7 [Eimeria maxima]
MRSFGAISAILAFCLSSAEANSGLKWVTWPENGSIKFSTPAAPQRLAVDVVDCMKVDVNFPENLVKVELP
SGESLELTSGERLQPNGGQLVVLFSSFGGQGHVIVNGEDVAGGFDTRMSSFAVPECFISRVRVTSPDGGE
LPSESLVAISEEIVNNFKKSYGAQSVNVEQSA
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Huang et al., 2015)
|
|
7. TFP250 |
-
Gene Name :
TFP250
-
Sequence Strain (Species/Organism) :
Eimeria maxima
-
NCBI Protein GI :
AAO52676
-
Other Database IDs :
CDD:294047
CDD:284955
CDD:238011
CDD:289699
CDD:214542
CDD:301595
-
Taxonomy ID :
5804
-
Protein Name :
TFP250
-
Protein pI :
3.92
-
Protein Weight :
238316.96
-
Protein Length :
2431
-
Protein Note :
Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of...; cl00057
-
Protein Sequence : Show Sequence
>AAO52676.1 TFP250 [Eimeria maxima]
MLHRNPRWALCAALAALYGGTGIASAEVNNELSKCESGWTPWTTCNPQTGLRERHNAQCETWVEVEECQK
LTGCGNWTPWSPGDMSCVVGQFQTRNREGCPEVQEVRACRPVLLECNDQWTPWTMCDTNRVQERYNSKCG
PVEVRECNMDDAEIEKCGEFVEWDPPMNGDCVRGGTHTRYRQNCPDRKEVRVCGAFDCSSCSVNATCDPI
GASCECKPGFRGNGKTCEAFNPCEDTPAPCDSNAICTPDGNDAKCQCKAGWDADSGAGSSKKPCVEVDEC
ASNTHQCPAHSTCINTKGSYKCDCNQGYVKGEDGQCHDVDECTNGEHTCPAHSTCLNTAGSYECRCDTGY
SGNATADSPCKNIDECANPNACSANAICTDTDGSFTCSCPEGYSGQGTHDSPCSKIDFCADPSLNTCGAH
STCVNTLTSFKCICDAGYEGAGTRESPCVDVNECSNEKPTNNCNRNANCTNTEGSYTCECKPGFSGDGMG
PNGCTDIDECAAEQSPCDPHASCSNTEGSYVCTCNTGYEPASTDGHACKDIDECATGAAGCHVSAQCLNT
DGSYECKCLEGFVGDGKTCNDVDECAAATSPCGDNTHCQNTIGSYECECKAGYGNMQDNACSDIDECKDA
NTKIPDNCLCVNNDGSYSLEAKAGYELVNGECIKIDFCARGACNSLASCKENEEGTAAICTCLPGYSGDG
TAEGHCNDIDECAGQNDCAPAEQGGICENTVGSYTCKCKEGYRQDGNSCTEIDECAEGTHNCHPSATCSN
TPGSFTCQCNSGFTGSGVECEDIDECSTEADDCGANTICSNTIGAFECNCREGYERADAKTCVDIDECAT
GTHTCSNHATCTNTDGSFTCQCNPGFEGDGHKCEDIDFCGAGQHDCNVHAECSESEDNTTFKCTCITGYA
GDGHGEAGCQDIDECAEENICGSNAVCTNTAGSYQCACREGFVASAEQQQQGTPALVCVDVDECSDASKN
TCAKPADGGICTNTEGSYECACKPGYQGDGHSCADINECTAQGTCGEHTTCKNTPGSFQCDCVEGFERAD
ERTCRDINECETGAVVLPPNSTCVNTEGSYDFDCVAGYRRTDGACVKIDFCKEKGCNANATCRENDAGTE
AICTCKEGYEGSGEGEDGCQNINECERGEPCKDFGEGGVCVDTPGSFTCECAAGFIQRRSVCQDVDECLD
GKLNTCAATGGVCSNTVGSFTCSCASGFEGDGHTCNDVDECATAQHTCDPNATCVNTEGSFECRCNAGFE
GDGHTCADIDECADPAKNTCDTHKGVCQNTTGSYTCGCKTGFSLAADGSTCENVDECAAGTANCNERSFC
KDTEGSYQCECKNGYKAAGEDCVDVDECEAGVHGCSEHAICTNTDGSYSCECMEGYQGDGKACEKTVGVC
DSAPCGAHATCEPAGDNYTCTCHPGYEMREGACVDIDECTAGSLNCDPHAICTNTDGSFTCVCGSGYTGL
GTSCEDIDECAGNAAGCDIHAVCTNTPGSFKCECKSGFEGDGTQCTEKVLLPGQIHCEAWTAWTECTDGA
KTSTRSCLALPLKKEMRACPAADFSQCGEFTEWTACPGTNNNLSHRRTERFGEPGCEDAEEVRECPDEET
EQKCGAWGEWTACGDPSPGLRTRARENCPDVVEFERCTMPSEPEAGEVTEPHTEGGAGVGGEVTEPDTEE
GAGVGGEVQPGTEEGAGVGGEVQPGTEEGAGVGGEVQPGTEEGAGVGGEVQPGTEEGAGIGGEVTEPDTE
GGAGVSGEPTEEEGTESTGPCKEFGPWTACKEDENGVGIQRRMCAGREDIIESRICTVTDDCGEWTPWST
CTNGSQARNKRFCTNVREVRLCGADIPVTDGCTWSEWTSCSLVNEEGGYFRTRTSSDCNMNEVQACSPSS
STTADSETEGTCSAWNPWTECSNGHQTRKCATMEAEESRTCGETPENCGEFGPFEPANCTAGQMVTRTRT
CGETEQKETKLCDVSSTEEGKQCGQWGPWSECNIHLGSEDNVRVREDTACGVTEYEECSKPANNAFVCTP
WSECSDKKERRTCTIRKNGLVQTRQEFRTCSVDIATTCGDFGAWSECNAEGLHQRSLEKCPDVIEVATCG
SEDCPPFGEWTECGVPEEGMRSRQRIDCVESAACQCTEVESCFDTELHPIPAPGTETGEGEGETETGEGE
TGEAGGEEGEQTGEGEVQPPEEELPGESVTEPEEKPEEELPEEEVTEPEEKPEEGVTQPEETPEQPVEGT
EEEGKQESEAAPETPAVQPKPEEGHERPEPEEEEEKKEEGGGFPTAAVAGGVGGVLLIAAVGGGVAAFTS
GGGGAGAQEAEQVEFEGEDTGAATAETPEADTVIDITDEDDYWADSGDIQ
-
Molecule Role :
Protective antigen
-
Molecule Role Annotation :
(Witcombe et al., 2004)
|
| III. Vaccine Related Host Genes |
|
1. IL15 |
-
Gene Name :
IL15
-
Sequence Strain (Species/Organism) :
Gallus gallus
-
NCBI Gene ID :
395258
-
NCBI Protein GI :
45383037
-
Genbank Accession :
AJ416937
-
Protein Accession :
NP_989902
-
Taxonomy ID :
9031
-
Chromosome No :
4
-
Gene Starting Position :
29463695
-
Gene Ending Position :
29495838
-
Gene Strand (Orientation) :
+
-
Protein Name :
interleukin 15
-
Protein pI :
8.34
-
Protein Weight :
21070.54
-
Protein Length :
187
-
DNA Sequence : Show Sequence
>gi|358485508:29463695-29495838 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 4, Gallus_gallus-4.0, whole genome shotgun sequence
CACCAGTGCAAACTTTATCCTAGTTTCCGTACCAACTTGCTCCATAGGTTTCCGAGGCTTGTAAGTATTT
TACAAACTTTAACATCCTCTTGCTTTTTTAAAAAATTTCTTTCTTGAATTAAAAGAATGAGGGAATTTAT
ACTGTTTAGCTTCAATTATTTTTTTTTAGTTGCCTTTTAAGACTGTTATAAATGTAAGAGACATACTTCA
AGTATATCTGACCACCTACCTGGTGTCTCTTTCACCATTTCTCCTGTAGGAGAGGCATTAGATATTATGG
AAAATTGGTGCTAGTCAGTGAAAATTTGTCACTGTTTATTAACAAACTCACAAAGCAAAGGAGGAAAAAA
AGACATTTCTGTGAGACTAGTGAACATAGCAAAGAGAGAGAGAGAAAAGACTGGGAGAAAAATTATTTAC
CAACCTGATGTATACTCTTGAAAGAACCATAAATATATGTATGGGACTGAAAACGGCAGTCAAGTGGAAT
CCTACTGAAAATGGTGTTATGGAGACAGAGTAGCTGATAGCAATGTACTATGAAAGTATTATCTGGTTAT
TAAGCCAACTAAACAATTGCATTCATGAATTTGCTGTTCAAATAACACTTTCTGCAAAATGAAATCCAAA
AGGAAGGAGGAAAAGGACATGTAGGGGAGGAACTTGTGAAGGACTGTGATTTTAAGACCTGCAAAATTAC
CTCTGTAAAAGCCAAAAATTCTCCTAACTGTGTATGTACATAATATTTATGATTTCTTCTATATACAGCA
GATATATATGTGCTGCTTTCCTTTAAAACAGTGTGATATTGCAGGAGAAATTCCTGATTATCATTACTTC
ATTATGTTTTGCTGTACTTTCCAGATAGACAATAGCACAAAAGTTCCTTTGCCCATTATAAATGTGTTCA
TTCCTACTTACAGTTACTAATTTATTTGAAATGTGCTAGCCAAATGGAAAGGTACCACTTAGTTATTTTC
TCTTGGAATGATATATATAAACATAGATCGACTTAGTATAAACAAACAGAGGTATAGAATTACTTCCTTT
GGAGTCTATTGGACTGTTCCGAACTCCAAGAATATAAGCAACTTTATAGCCTATCATAATTACTTTAGCT
TTGCGGATTCAAACAATAAGGATCACCTTCGCCAAATCCTCAGAGTGTAACATTATCTATCTCACTGCCT
TTAAAAGTGTTTGAACTCCCTGGGGAAAAAGAAAATTATTGACAATTCCACTCTAAGGTGTCCCAGACTT
TGACTGAAAATAGAAAAGGATTGCAGAGAACAGAGACACGGGCTGATGTGGAGTTATTGGGTAGTATAAA
TAATATGCTAGATTTTTTTCTGACGGATTAGATGTCCCATCTCACTTCAGCTTGCTGTTACGCGCCTTTG
CATGAACATTTTCAAGGCTGTGGCCAGTAATATATTTAATACAGACTTTCTACTGTCAGGTGCACAGTTC
TCACAAATACCTTTCTCTGTGTTCATCTCAGAATGTTCAGCTTTATGCAGCCAGCACAGGAAGGAGCAGA
AATATGTGGGAACTGTTCCTCCGTGTTCTGTCAAAAATGGTGTAAAATAGATCATTTCCACAGGGAGAGG
CTAGCACAAGGTATGACTTTCAGGAATAAGGAAAGGCTCAATAAAAGACAGCAGCCCAACCTCAACTGTT
GGAATAAATTTGTTAGTCTGTGCATAAGTACATTTTTTATCCCTATTGTTTTTCTACACGACAAGGTCTT
GTTGCTTGTAACTGGTACATGGTAACTGTTTTTTGGTTGCTGTCTTAATAAAAACAAATTTTATCTATGA
GATCTTTTAGGACAAATTGGAATTATTTTTAGGAGCTGTTAAATTCTGAGTCTCCTAATCTTGATGAGGC
AAATCTGGCTATTTTTTCTATTACAATTTTATGATGTTATTACCCATAAAGGTACACGCATTAACAAAAT
TGTTCAATATCACTGTCATAGACAGATCATTACTAGTGATGATCTTTTCTGCACAGGCCATCATGACACG
GTAAAACATACTGTGCCTGAAAATGGAAATCCAAGCTGTTATCACAAGTCTATAAATACTTCTTGGCTCT
CAGTGTGAAGTTGTAGTGAGTTGTATACAACTGAGGTAGCAGGCAAAAATGTCACTGCTTGGGATGGCAA
GCTGCCTTCTGTGTTTGTCAGTCCCTAGCCTGCCTTTTAAATTACAGACGTGACTTTCCCTGAGTTTCTG
AAGTTTGAAAATTGCTCAGAAATGGAGAGGACAAGTCCCTACCATCTGTTATTTGTTATCTGCTATTTAT
TACTCTGAGAAATGTATATGAAGCACTTATTTATATATAACTTATATTTTATAAGTACTTCCAAAATTTT
AATTTTTTATTTTCACACAGCCCATATTTCCAGTTTCCATTCTACAGATTTTGCTAAGACAGCAGATGAC
TCTGGGAATAAATGCTGACATATTTTCAAAGTTGGCACACTTCTCTCTGCAAGGACCAAAGTTGAGTACA
AAGCACTGCTAGCGGATCATGGGAAAATTTGCTCAAAGGTCCCCTTTAAGCAGGAGTTCAAAGAAATGAA
ATTATATAGCAAGTCCGCAGTACCTCAACACACAGATTATTCTTTCATAGAAAAAGGGGCCTCCAATTAT
ATGTAACTGATCCCAGAACAAACTTACTCTTTCACCATTTCCTTGCTGAACAATGAACCTTAGTTGCTGA
TTTCAAAGGTTCCTGAATAGTGAAGGACCTTCTGCTCTTCTTCAGCCAGTGCTGTTACGAAAGCTGGTGC
TATGGTCCCATGCTCAGAATGAATCTGAAGGAAGAAACATTGCAAAAAGATATAATTTTCATCAAAGCTG
TCTGTACTATCTCAGTGTAAGCATAGATCTCACATAGACAGGATCACCTTTTAGATAATATTCTATAATT
CTCTGTGAGAGGTCTGGGCTGGGACTTCACTTTAGCTCCATCAGGATTCATGGGTAAAATAAGGCTAGTT
CTTCAAGAAAATGATTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT
CTCTCTTTTTTTTTTTTTTTTTGTGTGTGTGTGTTTGTGTGTGTGTGTGAAAACAAAAACAAAACAAAAC
AAAAAAAACAAGGGAAGAGCAGAAGTATTTGCAAGCAATTTATTTGCCAGCAGGAGGTTTAGCATATAAT
TAAACTGTTGTCCTTGCTGGTAGGTTATTACCAGCAGGTAACAAACCATCTTACGGTATTTCGTGCTATA
ACAGATGAGATAGGTCTTTAATGGTAGCAAAGGGTGATCACTTAGAAATTGTTGTGACTATAGCTGCTAT
AAATTGCGATCTATAGTTTAATAGTTCAACAATTAACTCTACCACTGACACTCAGAAGCCCTTTTATAAA
ATCCTCTGATTATCCTCCCTGTCCCTCTGTGGAACTGTTTTTGAAAACCTGCTTTATAAAAATACCTTTA
GACTATAAGGTTAGACTCTATGACAGATAAAAGTCTTATATCAAAGTTGCTAGACCAATACAGCCATGAC
TAGAATTTGTTGTATGTTTGGAGTGCCTTTTTAAATATAAGAAAAAGGCCCTTTCATAAATAAAAATTCA
AATGAAACAAATATACTGAATGCAAATTGTTTTTGAGTAGAGAGAGTGCCATACAGTGAAGAACTAGCCA
ACAATGTCAATTACTGCACCTTTCTCTGCCTGTAATACCTGCCAGAAGGCTGTGAGCATGAAATGCTCGG
TAATTTTCTTCCCTACAGTTGAAAAAACAGCTCAGGATGGTGTGGAGAAGTTTGTTGTCTATGCCGAGTC
AACAATTTGAGCTCATTGGTAATGTCAGATTCTTGTCTCCCACAAAAACCATATGTGCAGATAAAAAATA
TGACAATGCTGTTTTTGACGTAATGATCTTGCAAACAAATGATATTGTAGAAGATTCATTTTGTGTACAG
AAATAACAACACTGACAAAATTTCACTCCACATATCCTAAATGTCATATAACTGTTAATATGCTGTTAAC
TACTATTAGCATATTATCAGTTTTGTACTCAACATGATGGAAAATTGGGCACAACCTCAGCTGCCTTTCT
TGATGCCACGCTGGCTAGTTAAGAATTACAGCACCTACACCAGTAGTGCTTGCTAGTACCTCTAGCAAGT
GACAAAGTGTCGTCTTCCTAGAAGGGCAAATAAGAACAGGATTAGGGGAAGGTATTGCAGGAACATGGAA
ATCTATGCTCCTGACATCTGAAAGCTGCAGCTGGTTACTGCGATCAAACTCCTTGCAAGAGAAATAGGTA
TCTGCATCTCTCACATGGCACATGCAGGACAGACAAGTTATCAGAAGTAGACAGTCTTTGAAAGACAGGT
CCTGCCTGATTGAACTTATCTCCTATGACAAGATGACTTGCTTAGTAGATAAGGCAAAGGCTATGGATGT
TTTTTATCTCAAGCAGCATGCCAAGTGGCCGAGAAAGCCAATGACTCCTGACCTGCATCAGAAATACAGT
GGCTAGCATGACTGGGAAAATGATTTTTCCCTTGTACATGCAAATAGTAAGACCTTACATTGCATTCAGT
CTTGGGCCCCTCACTGCAAGAAGGACATTGAATTCCTTGAGGGTGTACAAAGAGCAACAAAGCTGGTGAA
GGGACTAGAAAACAAAACTTACTAGGAATGGCTGAGGGAACTGAGGTTGTTTGGTCTAGAGAAGAGACTG
AGAGGAGATCTCACTGGTCTCCACAAGGAGAGCTGCATCAAGGAGGGTGCCAGTCTCTTTTCTCAGGTGC
TGAGTGAGACTACATAAAGAAATGGTCTCAAGTTGTGCCTGTAGAGATTTAGATTGAACATTAAGAATTT
CTTTACAGAGAAGGTGCACAAGCACTGAAATGGGCTTCCCAGTGAATGGTGGAGTCTCCTATCCCTGGAA
GTGTTTAAGTGTGGACATGGCACTAACAGACATGGTTTAGTGGTGGGACTCAATAGGTCAGGCTGATGAT
TGGACTTGATCTTGAAGGTCTTTTCTAACCTAGATGACTAAGTGATTCTATGAATGATGTATTCCCAGCT
TGAATGTTATATTAGATTGACTAAATTTTAAAACAACAGGCACCTGCATACCTGTTTAAACTGGGTGCAT
GTAACATGCTTCTAAGAATGTGGGGATGGGAGTCGTAATAATCTCCTGCAGGCTCATCTACTGTAGCCTG
TGGTGGAAAACAGAAAGTATGGTCTGTTTCCTTGCCTAATGTAGTTTGGTAAGGTTGTAGATGCTAGGTA
GAGTTTTAATTTATGCTGATTCTTGGAAGAGTCATTGGATCAAATAGCGTTTCACATTCCTGGAGCAGAA
TAAATTCTGATATAACAGTTAAAATAATTGGCTGTACTTTGGGCTGCTATGTGCAGCACAGATTACCAAT
ATACAGCAGAGGGGATGAGATAGAAATTGGCACTGAGTGTCCACCGGGGGTTGCACCGGTCCCTCTTTTC
AAGTCAGGCTGGGGATTCTGGAAGTTGACTTGATGTTGTGATGGCCAACACCAGACAATGGTGATGGCAA
AAGCCAAAGGAATTGGTCTTTACATGTTGCCCGACTGCTTACATCTTTCTTCCATTAATTTTATTTAATT
TTACATCATTTTTTGTTTAATTGTAAAGAAGTCTACAGCCTAAGTATCCTTTCTATTTTATAATATAGTA
ATTAGTTACATGGGCACAAGAAGTAAGACTGTACTCTGACTAATATATATACAGTAGAAAAATTAGATAT
TCTTCTTTTGCACATCCTCATATCTGATTTGCTGCACCTTCCTTGCAATGTGTTGTAAATGTAGAATAAT
TAATTTTATCTTTTTTTTTTTTTTGCCTTCCATTTATAGCACTCAGCAGTACAATAACAAATTGCTTCTT
GAACATGAATCAGTTCATTTTCTCAAAAGCCCTCAAGGATAATGAAATGGGAATTTTATTTTATAAGTGA
AAAAAAAAAATCTGAAGAATCTAGTGATTTGGGGTCTGGTATGTGATACCTGGAGATGAATTTTTTTAAG
AGACTGTTAAGCATTCTCGAGGATTGTCTCTGTCTGTATATAGCTCCCAATCTGTTCTGTTGCTGCTGTG
ATAACTCAATTATTTTTTGAATTAAGATCTGTAGAGGGCACTTATTTCTTCAAACTGTAACCACCTTCAT
TGGAGAAACCTCCATCTGAGCTAGCTGACTGTAAGGACTAGAAATATATGTTTGGGGAGCATGAGGCAAG
TCATCCTAAAGGCAGGCACACAAAGCAACTTAAAACCAAAATTCCATGTAGGAGGGAAATCACAGAATCA
TCAAGGTTGGGAAAGACCTCCAAGATCATCTAGTCCAACCATCTGAACGATTGATGCTTGTGAAAAATCT
GGGTTAAGTAACTTGCCCAAATCAAATGGAGATGGTAAGATGAAGTCAAGGTTGGGATTGTTGCTCAGAG
CTGCTAAACTCAACTGCTCTTGTCATTGAACTTACATTTTTCTTTCTGTTATCTTCTGCTTCCTTTTGTA
TACATCTTCTTTTTTCAAGTGAAGCGGTTGATACAGTCTCTTTCACAAGATTGGCCTGTTCAGCAAACAG
GTTCTGGTCAGACCTGCTTCTGGTCATTCTGGTCAGAAATCCTGTGAAAGAAAAAGGAAATTATTATACC
ATACTATCATTAAAGACTTGGTAACTTTTTAAAGTTATGAATTCACAGGAAGGTAAGGAGAGTAGGATTC
AGTTTCTGTTTCTGGAGAAGAATATACTTCCGTGATCATAAATCCTCCCACTTCTGTTACAACAAAACTT
TCCTCCTTCCTTTCACCTCCTCTCTCTTATGTATGCCCTCCATTCAGCCTGTTACTCAAATTCCTCTGTT
GTTTTGCAAACACCATCACTGATTTCTGTTCCCTGGCTTTCACCACATCATTCCTGCAGGAAGCCTTTTC
CTGCAGGATACCTTAAGTTCTGCTTGCCTCTGAAGATCTGAGATGGAGTTTGTTCACTGTGAAGAGACTG
CTGGAGACCAAATAATCATTGCCAAGAAATCTCTTCTGTAATTACGTGAACTAACCACTTGTGACTTTGC
AAAATTTGTGCAGATTTTCTAGGCTGCCACATAGAACAAATTACTAAAAAGCTTTAGATTACTAAATTAC
TAAAAGAGCTTTAATTAGATGGTTATAAACATTTTCTTTAGTTCTTTTAGTTCTTTTATTTGCTCATTTG
TTTTTGGAAATGACTGAATTATGTTTGATAAGACTTTCAGTATAGGAAATTTCAGATCTAACAATTTCAT
CTTGACCAAGATGAAAGTGACCAAAACAGTTTTCTTGTTTGAAACACAGCCACAGAAGGCCCTAACAAAA
CTGTAGGCAATATCACCTTTACTTGAATAGAAATAGGACTGTTTCCCCAGTCTATACATTTGAAATTATC
AGAGTTTAATTTCCTAAGAAAATGACATTTATCCAATCACACATCTGTGCATGTTTTTATATTTTTGCTT
CCCTCCTCTATCACATTAGTTTTTAATCCAACACCTAATTTCATTGAAGAATTTATATTGGAAGAGGTAT
CTAAAGATCTTTTCTCCCAGCTTCTCAACTAAAGCAGGGACATTAGTGTAGGTTGCCCCAGATTTGGCTA
AGTTTTGAATACTTCTAAGGATGGAACTTCCACAACTTCTCTAATCAGCCTCCCTTGTTGTATAACTGCC
CTCACTGTGACTTTTTCCCCTCATATCACTGTTTTCTCCATTTCTTTTATTGTATATCCCTCGTAACAGT
TTATATCTCTCTTCTCTATAACCTCTCTATGTAGTGGAAAAAAAAACAAGATCTTCTTTGCCTTCTCTTC
TCCATGTTGCACAAACCGAAGTCCCTTAGCCTGTGTGTTCAGTCTCCAAACAATTTAGTGCCAGACATTC
CAGTTTTGCAGTTTCTCTTGTACAGTGGGGTTAAAACTGTACCTAGCACTTCAGATATAGCCTGTATCAT
GTAGAGCACCACTTCAGAAACATCATGGATTTTTTGATCCCTTCATCTTGACTTTCCATCAGATGCTGGG
TAGTTTAATCCAGTCTGCAAATGTGAGACTTTTTCCATTTGTTTAAAGACAGCTTCATTCATTTTGATTA
TCAAGCATCCTTCAGCAGACCCTCACTCTCACATCCTCATGCTGATTCCTCCTTTCATCAGGACTCACAC
TCCTTGTACACCAGAGTTTATTGCACTAGAGCTTTGCACCCTCTGGTCTTCTCTGCTCATCTTTCCTTGC
AAGTCTGTACTGAGCAATCGCAGCACACTGGTGCTGCGATGTCCCACCACATCTTCTTAATGCTAACAAG
GCCATTGTCCTGTGTTTATGTGAACTGACAATTGCTCCCACTTCTTTCACAAGGTGGCACGCATAACTAT
ACAGGTACTTCAGGTGGACTCTCAACTGCTCTGTTTAAACAAGTGCAGTTTAAAAGCCTTTCTCATCACC
CTGTTCCATAAATTCCCAGCCTGGAAAAGCTTAAATCAGGCTTAAATCTAAGAAACCAAGTCCATTTATG
AGACAAATTAAAACCAGACATTGCAACTTCACAGGTCATCTGTACAATATGAAAAACAGTAAATAAATTA
GTAGCTTTGGAGATCTATTTTCTTGCAGATTACGTTGACTGCTTTCATCAAAAATACAAATATTAATATT
CAGATAAATGGTACTGTCAAAGTGTCTTCATATTTTTCTGCTGTATGTCTTCAAGGTCTTGCTGTGAATA
CAAAATTCGCTGTTTTAACAAAAGAAGTTGCTTGTTTCTCCAATCCCAGCACCTGTAAGACTTTAAAGTG
AATGTAGAAAAAAAGTTATAAAGGAGAAGGAAAATGTGCTAGTAAGTTTTTATCATTTCATCTATAAAAA
GATAATAAAAAGGGGAATGAGCTAGTTAGCTGACATGATACCTGCTATGAAAACACTGCTTAAAAGAAGT
GGCATACTGGATTGCTGCTGACCTGACCTTGTTTCTATATGGTTTGCCTCATTTATTCTCTTCTAATTTA
CCTCGTAAAATAGAGGAGAATGAAAGTAAAATTGCATAGCTGGACTGACACATAAAAAGGAACTATTAAA
AACAAGTGTCATGCTCTGTTGATGTAATTAGGGGAACCCTTGTAGCCCTTTAGAAACCACAGAACTATGC
TGATGTTAATGACTTTCATATTAGCGAATTTAGCTGCAACTTCTGTTCTAATTTTCATCAGAAGTAATAA
AAAATTATCTGATGACAATATATCTCTTCCAGAGTGACTCTCCTCCCTTATATTGCAGTGTTCAGAGATT
CAGTCTAAAATGGGCAGCTGGTCTCCAGATAAAAGTAATCCTTCTGAAAAGACACATCTGGTATTACTAT
GAAAGTCCCATGTAAAAGTTCAGCTCAATGGTTGGAGTGCTCTTGACAGACCATCTGAGAGCCACTTTGA
GCTTTATTGCAGCATTAAATACATACTTTTTTTTCACTTCTAGGTACCGCAATGTATTTCCCGATCCAGA
TTCTGTTCTTCTGTTCTGAGTGATGTTACAGTTAACAAGATGCTGGGGATGGCACAGCCAACACAAAACT
CTGCCGGAGCACGGAGAAGGCCGGAGAGTCAGGTATGTGTCATTTAAACAGTCTGACATTTTATGCAATG
TCTGCAAAGCTCTTAGTGTTACTTACATAGTATAACAAGAAAAACTGTTAATACAGCAAATTTAAAGCTT
GGAAATATTTCGAGTATTTTAGACAAGACCGCTAGCACTCCACAATTTACTATTGTGTTCTTTTCACACT
GTTTTCTATTTATTCCATTCTCGGGTCTGGATTTTTACAGACCCGTATTATTTTTTTTCTCCACAGCTAA
ATTCTAGCTGACATTCTGCAACTTGTGAGTCTGTAACTCCTGACCCCCAAGTGTGTTCAATATAGGAGGA
TAATTGCAAGTGTTCAGCCTTTCTAGCACTCTTCAGAGCTTCTCAAAACACTTTCTGGATGATTCAGTGT
CAGTTGTATCTCTAGTAAGCAGCATGACCATGTGGCCTCCTAATAATCCACAAGGTGTTAGCACATGGAA
CTCAGCATCCCCAAGGCTTGCTACAGCTCCCTGCAGAGAGGCTTGGATACCTGTATACACACTCACCTGG
CCATGCCCTGCCAACTCTGTCAGCCTCCTCTGGGAGCTGGACTGCTAGGTGGCCGAAATATAGCAGCCTT
CTCTACACACTGGAGGTTACCAGAAAGAAAACAGTGAGTGCTCCAGCTGTGGACACTCAATTGTGCAAAG
GCTGGAGCACGTATCTCAGGCAGTCAACGGGCATAGCCCTATCTGACCCCACCTTTCCTACCACTGTGCT
ATAATGTAGACAGACACATCCCCAAGTAAAGCCAATCTGCCTAAATATGCAGGTTGTGGGGGGAAAATGC
CACACAGGAATTGTTTGAATGGTCTTCTTGATGATTATAGCAGTGAAGTTGCTGCTTAGGGTGATTCCTT
TTACAAGAATTTTTGATTCTTTTGCTGTTTATCTCAAAGCTTCTAGATAAAACTCTTCCATTCTAATTAC
ATCATAGACCTGGCCCCTAGTAGAAACAGGAAGAATACAGCTCACATGATTATGGGGAAAAATTTGGAAA
TCGAAGTTCACCACTTTAGCTCAACAGGACATGAGGAGGATTAGAGCACATAATCCCAGTTTATGGGACA
GGTGAAAGATACAGCATAGCTAAGTCATGCATATAGTCATGAGGTTAAAATAGTAAAAATATTTCCATGT
TTATTGACTGGTATTTATTGTACCTGCAGCTGTGTCATTTCTCATTCATTTGAATCATAGTAAATTTTCC
TACTGGAACAATAAATTTCATTATTAGCTTCATCTAAACCAAAGAACTTGAAAGCTCAAATTTTAGCATG
CCTCTTGAAAGTCCCAGTTAAAGGGAAACTTTGGGTGATACTGTTTCTAAAGCAGCTCTGCCAATCTCAA
ATATTTTTCTTTAGGGATCCAAGGGCCATCTGGTCAGGGCCTCATCAGCCAGGGCCTATCCTACTAACCC
ATGGAGGGAGCAGGACAAGCCACCAAGGCAGCCAGGACAGACCAGAGTCCCAGTGACAAACTAAGTCCAC
AGTGAGAAAGTCCAGAATCAGGCTAGAGTTTTGAGTAAGCAGGTCAGTAACAGGTGCAGTGATGGTGATC
AAGAGGTCTACAATGGTAGCCAGAGAAGACCACAGTAACAGAGAGATTTGGGGTCAAACCAGGTAGTCCA
TCCACTGGCCAGGGTCACATCAACAGTACAAAAAGCCAGGCACCGGCATGAATCCAAAGAGTCAGCACCC
AGGGCCCCAGTGCTTACAACCTTTGCTAGAGTCATAGCAATTTGCCAACACGGTATATTTTTCATAAGCT
GAAAAAGTCACTTGTTCTAAGGCTTGATTCTTGCATTTATCTAATTTCTTACAATTCTGCTATTTACATT
CTCTTGGGAGACTGTGGTAAAAGACAAACAATCGTGCTCAGGTGATCATAGTACCTTTCTCCTGTTGGAT
AGATTCAGGAGTACTGTAAGCTAGAATGATGGCACTAAACCTTTTAAAAACTCATCTTGTTACAGTGCTC
TCAGTTCTAAATCTCCTACCTTCTGGTCTACACTTCTTATCTATTTTCTTATCATATTCCAACTCTTCAC
ACGAGACTGTAACTTCCTGAGCCACTGACTACATGTTCAGTCTCCTGCCCACTCTCTCCTACAAAAGTGC
AGTTCCTAAAACTTTGCAGATGGTGCTAAAATACCATATTATACATGACAGTGACATAACAGGTCAGAAG
CCAGTTACGTGCAGAGGCAGGTTAGTTTCTCTGCAGTGACTGCAGGTGACACATGAGTCGAGTCAAGGTT
ATTAGCTACTCTAGGCCTGTAAAAAATTTCAAATTAATCATAAGTAAATGTTTTTTAAATTGATGCAGAT
CATTTCAAATACAAATGCAATCAGTGTTATTTCCCCCACTGCTCTTAAAGAATATACAATGCTGTACTAA
GTCTGTCTGGGATAGAGTTAACTTTCTCCACAGCAGCCCACAGAAGCTGTGCTCTGCACCTGCAGTTATA
ACAGCCTTGATATCACACTGATGTTTTAGGCTTCGCTGGCATCCTACCAAGGCTGGATCTTTATGCCCTG
TGCCCAAAGGCCAGTAGGCTGGATGTGCAAGATGTGCAGAGCAGGCACAACCAAGGCAGCTGGCCTAAGC
TGGCTAAAGGGATATTCCATAGCATATAACAGTACACTTAGCTATAAAAGTTGGGGAAGGAGAAGAATTA
TGAAAGTGTTGGTATGCCTAAGCAATCGCTAAACATATTGAGGTCCTGCTCCTGGAGATGTAGCTCAATA
TCACTTGCTGATGGGAAATAAAGAATAATTATTTTTTCCCTTTGCTTCTGCATGGCATTTGTTTTGGTTT
TGGTTTTGTTTTATTATTACATTTTTTCCATTTCCATTTAATTAAACTGTATCCCCACCTGCAAGCCTGT
CTGGTTTTCACTGTTTTTACTCCCCTGTCCTTCTGTGGAGGGAAAGTGAAGAGCAGCATGGTAAAACTTA
GCTGCCCATTGATTGTTAAAACGCTACAAAAGCCATTCATAGTTCATGTGGATATAGGACACTGTGATTA
CCACAGTAATCAAGCTTGTTTTGCCCTTTGGACACAATAATGTTGATTCTGTTGTAACTTATCAACTTCA
TCTCCTTCATTCTTCAAGATAACTTAAGAGCTTTCATGTATTCTGGGAATGATTCTATTTGGTTCCAAAG
TCAGTGAACATTTTCAAATATAACTGTACCATTGCTCTCTTTTAAAGGTATATGGAAGTTCATTTTTATA
GCATTTACGCCATTACTTCATCATCACTGAAATTAATCATTTGATGATATTGTTCATATTTATACAAACT
GATGTAGCCATGTAGGTATAAACAAAGGAAATGATCTTTCCTGATATAATTTGCTATCGAAAAATGGATG
TAACATATTTCATTAACATGTATGTTTCAGTTGTTAAAATTAAAAGTGCAGATGAGTTAAAATAGTAAAT
CACAGGGTAACCATCTACTATAAGAAAAAAAGATAAAGGGATTGCACCCCTGGAAGATATAATAGTAGTA
CTTTTATATAAGGATTTTTTTTGCTTTAGTTCTTCAAATGATCATTAAAGATTTAAACTTGGTGATATTT
CTGTGGCTGAGCTGTCTTCTCACTTAAAATACTAGCTTAGAAATTACTGCACAAAATGTAAGAGTATTCT
GTTGTTAGAAGTTTCAAAACAGTCTCTAGAGAACAGCTCAGAGAGGGATCACTCACTGAAGAACAGTCTG
TTTGTTACAACAACACTCGAAAGAAATAAAGGAACCAAAGTTAACAATCTGATTTCCAGAAATCAGACCA
AGTTTCCATTATCAAAATAACTTTGAGCAACTAAATCCATCTATTGGCTACTTTACTATGAGTTATGCTA
GAAGAAAAGGTGCTCTTTAAAAAAGAAGTATTGAATAGCTGGTCTTTGGGAAGTTGACAGGGTTGACAGG
CAATGTTATTAACCTACAGGGTCACAAGTAATTACTTACCCTGATTGTGTGTAAGGGTGCTTCTGCATGT
AACTGGACTGTCTTCTGTCCACTGTGGCCTGTAGTTTGTGTGCTTCTGTTTGGAACTGTTGAAGACAGTG
ACTGTTAAGGAGATTTCTGAGAAAGACTGTAGTCCTAAAATTAAGTGTATTTCATCTGGTGTTGCGCTCC
ACTGATTCTGACTAGCAGCTACTAGCACAAGCAGAATTTTCTGATCCCTTTTAAGTTTCTATGATAGTTT
ACCATACAATTTGAAATTACACGTTTATATTAAATCACGTTAGCTGATTCACTATTCCTACTGTAAGGTT
CTCTGGCTGAATTTGAATTTTGAAATTGTTTAGACATTTCACCAAAATGTACTGTCAGGCATGTTTGCAG
AGCTGAACATGCATGTAACTTATTTCTGAATTGTAATTACACCAAGATTTACTGTTAAAAAGATCACTGA
AATGTCAACATTACATAAAATCAATTAACCCACTTATGGAAACTAAATATATCAGCCCCAATCTCATTAT
TTTTTCTTTTCTATGAAATATGTTACCATACCTGTTTCTTATTCGGTTGAATCTATTTATCTACTGAAGA
ACTCAGTGCATGCAACCCTTGACTTCTGCTGTTCAGGATACACAGTTCTGTGAAGTCCATGTCAATACCA
TTGAGAGTGCATACTTTTCTGTTTCCACCACTGCCTCCACTTTTGCTCTTCATCCTCAATACATAGAGCA
CCATCTTTTGTTAACACAATCAACTTTAGGTCTTTAACTACAAAACAAATGAAATGCGTTTTTATTTAGC
TAATTCGTGCTCGTAGTTTAATCATGTTTCATATGCAGCATTTTGTTACATCTTTGTAATTATCTACCCA
TTTCCTCTACTGTACTACAAGACTACCAAGGTTGGTTAGGAGAGGAAAGCCTTCAAAAGAGCTCCAGGGA
TATTCACTCCCTTGTTTCCCCTCAGAGTTATAAGACAAATACTAACCATGTAATTATTCAGTGCAAGAAA
TAAAATTTGTCAAAGTTCAAAAAAGAAAGAAATTAGAAGTGAGCATGGAAGTTAGTTTGCAACATCAGTG
AGCATTTCTGACTTACCACACGCTTTTAATTATATCCTCTTGGGAAGTGACTTAGTTAGGCTATAACAGG
GTGGACATGCTATGTGACAAACTTCCAAGAAAACCTAGACAGACCCAGGAGAGTGTTGTTCCATTTCATT
TCTGAACAAGTTGCCTGTTGGAGATACATGTCTAAGTTGCAGCTTTCCTCCAGTGTTAGGGTAGACTGCA
GTCTTGACTTGTTTTACCTTTACTCCAGGATGTCCAGCCTTCTCCCAACTCAATAGCCTAAGCCTGAATC
ATGTCTTTTTCTACAAAGGTGAATGAAGATGTGCTCAATCTACAGCCTCTCCAGATCATGAAAGGAACAA
AGGGAAAAATCATAGAACAAACATTCTAAAGCAGATGAAAGTACAACTTTTATCACAATTTCCAAGTAGA
AATTGATGTAATGCTGTTATTTTCGGTTTTCTAGATCCTGAGTAAGGGTAAAAAAATCTCTTCCACTGAG
GAGCATTTCCAATTTCAGAACTGACAAATGTATATAGAAAGATTAATACTCCCTTTCTTACTTGCTCAGT
TCACCACCCATAAAAAGAAGAAAGGAGCCTATATGAAAAAAAGTGACATGCATAGGACATCGTCAGAAGA
CAGTACTTAGAAACATAAGAGCATGCCGAAAAATCAGCTGGAACAACTGCAGAAATAGATGGTTCTCTTC
AAGACAATTTCAGTTAAAACAGCCTAGAAAAGTTTTCCAGACTAAAATTAATGACACTTGCTGACAGCAG
CTGTGCATATTTCTTCACTCAGGCCACAAGACGATGAATCATTGGCAACCCCACCTCTTTTTACTCTCGT
CTTTCCATACACATGCCAAGGACAGCAGAGCAAACTGCTGTCCTTACAAGTAGCATAGACTTACTAAAAC
CTGTGGCCTCAGTACTTGCAGGCAGGCTCACTATATTGGCGCTGCTGCATTTGGTGAAGTTAGGACCAAA
TTCATTGCAAGTGAAACAAGCACGGCTTTGGAAAAACTGCTGAAGCTTCCTGCTATTTTTTTCCATTCAT
TTTGAACCCGTGTGCCTGGCTTTTCTCACTTATGTTTAGCTTCTGCAGACCCAAAGAGGACAAGCAAAAG
CAGCTAGGAAAGTCAGAAAAATCTACATCTGATATATCACATCAGAAATGGTTACAAGGATCATTGTTTT
TTTTTTTTTTTTTTTTCACCACAAAACCCTCAAAATCATTAATATCCTGCTGAGATTCTGATATGCAGTA
TACTGATATTGAATTAAATTGTTGAAGAAAATTTACCCAGACCCAAAACAAGGTAGAATACAGCTCAATA
GATCTTATAGCTCTATAACACCTACAGATACATTTAGGTAAATTTAAGATTTTACCGAATCAGTCCAATG
TTCATTTTCTGCAGAAGTCGAAACATAAGTTTATCACAATGTACTCCTTTGTTCAATTTTTATAGTAAAA
TTTGAAGAAAATGATGTGAAAATATCTCCCTGATCTAGGGAGAAATTTTTACTGAAAACTTTATCTCAGA
AACACCTCATCCTTTTCCAGTGTAAAATTTTTCAAGATGTGGTGAAGCATTTAGGGTCTAGTACTCTTGA
ATGATGAGACAGACAGAGGAAGACTCTGGCCTAAGAGCGTTGTTTTATGAGGGGGTCAGGCTTATTTCTC
TCAACAGAAACATAGTGTATACATACACAGTGGGTGAAGAGTGATGTACGTGCTGGAGCTGACAAAGATG
GAGCTTTGTTATGGTTATCTGTTTAGCTGACCAGAACACAAAGTGTAGCTATCCTTTCTAACATTCAAAT
TTAACTTCAGCTTTGAAAAAGCAATCTACAGCAGACAGAAGTCCTGAGGATATAAACACCAGACAGTTTT
ACTCATTTTCTTACCCCCTTTCTTCTACTATTCTGTATTCAGAAAACACACGACTTGCTTATTGCGCTCA
AAAAACTCTATGCTAGTGACGATTGTTATCTCTGCTGGTGTTATCTAACAGTAACTGAATGCATTATAAA
AATATAGTGATACAAAAGAAACATCTAGAGCATGTACATTTACTGATACAGTTATTCTTCAAGTAGCTAT
TCAACTTTTTTTTCCATGGGAATATTTGCAAAACTTTTCCAGTGGTCTACAACAATCTACTTTTAAAGCA
GTTCCTTCTTTCTTTCTTTTTTTCAAGCTTTTCTATTACAGGACAAAAAATCTCACTCATCTCCTGCCTT
TGTGTAACTGAAAACACCTCTCAGATTCATGAGATTTTATGGAAGCCTGTAGATTATTTTGCATAATTTA
AATGGCAAATGAATATTTCAGCTCTCATTTTAAACTGTGTGTGATTACAAGACAAATAGGTCACAGTGAG
AACGAAGTGGTTTTCTAGTAGACTTCATCCAGTTAAAAACAAATGACTCGGCAATAATGCAAATTTATGC
ACTGTGCATACGTGACGATTTTCAACAAGAAACTGAGTGACATACTGCACTGAATGTTGACTAATGAATA
ACTGTTGAGGAGGTCAACTCAAGTTCTGCACTACACTTAGTAAAAGTGAGTTTTCAACACTAACATGACA
CAACAAGATGGAGGACTGGTAGCTATCCTATGTTCTTTTTAGGATCTTGTGTTGGGGACCTAAAGGCCCC
TGAATGAAGAATGATCCTGGGATGAAACAAGTATTCTGACAGTTTTAAGGGAAGTGGCTACAGCCACAAA
TGTTTTGTTTTGTAGAGAGAACTGACCTTGGTAGTTTATCCTGTTTCTGCAAAGACTTGCTTTTCAAGCA
GGTGTTCCACTTGGGCTCTGTATCGTGCATGGTATGGCGTGCAAGTGGAAAAAAACCCTGCAGACACTGG
GGAGAAAAAAAGGAGGAGAGAGTCTGCAGTGGGTGAGAGAGGAAGAAGTGCATCTTGAAGCAGGGGATGC
CCTGCTGCCTTCACCTATCAAATCCACTGCCAGAATGTTCCTCCTCTCCCCTTTTCAGCAATTTGCTGTT
TCTCAAGGGTCTCTAAGTAATAGTGCACTGGCTTAGGGAGTTTCTGTTTGCCACTTATTGGGATTATCTA
CAATGAATGCCTGCGTGTATATGAGTAAGCTGAATAAAACTCATAACCCTCTGTGCTGCTCTGTTTGTGC
ATAGTTTGCTTGCCTCTGGTCCTGAGGCATCCTGACTCTCAGATCCAACTGTACCTTGAAACAGATTTGA
AGGCTTTTACCAGAAGCTACACTAACAGTTCAGTGCTTATACTATACCAAAAGTATGGTAGCATCCTAGA
ACTAAGACAGAATGATTTATCTCAAAGCAATTTATGTCACCCATTTTATCCTGACTTAAATATCGAGTTA
CAACAAAAACTTTCATTTAAATCAGGGTTCTTAATTTTTATTTCTATTTACATACCTCAGTTACTTTCTT
AAGAAAAGGTCCACACTATCACTAAAATAAATTTTCAAATCAAAGAAATCTCCATATTAAACATACTAAG
AACATATATTTCTTAAGCAGTTCTGTAAGTTATACACTCAGAATCTTAACTGTTATCCTAGAAGCTTATG
AATGATGATTTTTTTTGTTTGTTGTTTTGTTTCTTATTTTGCTTAAAACTTCTGTCAGTATCTGTTCTGA
AGAAACGAGTACATAAATAACACACTTTAAAACAATTTGAATAATGTCTTGCATGAGTATCAAAAGTAAT
TCTGAAACAGAAGAAAAAAATACCACTCTCACAGTAGTAGATTATTCTTTTTGCTCAGTATCCCTCAGAG
TTTCTGAAAGACATGCAAGTACATTTAGAAAAATCAGCCTACCTATTTGAGTAGGATAAAAACCTCAATC
TTACTGAATTCAATGGGATATAGATATTATCACCGCTATAGCTGACCATAACTTCAGGTCTATGGGATGT
GACTTTTCTCTGGATCAGGGAGAATTCTGCAGGTACTTGTCTTGGTGACCAAAATAAGTACAGCACATTT
TCCCAGGTATGTAGAATTATGCCATTTTTTACTCACAGTTTACAACTAAGAGAGAAGAAACTTTGTTTTC
TCCTTTCCTAATAGTTTCTTCAGCTTTCACATGGCTATTTCAAATGATGAAAATGTTCTCACAACACTTT
ATGAACTGAAGCAAAAATAAATTAGACTGAAACCACAAAGAAAAAGAATATTTGTTTTTGGTTAGAGTAA
ATATGAGCATTTGTTGCACTGTAATGGCTGAAAATAATATGAATGTTTACTCAAAGTAGTGACATTATGA
CTTTCAGAAAGCTTAAGCTGCCCTTAAAAAAAAAAGATATGTTCCCTTAGATTATACATGCTTATATAAA
GCAGTACTCTTCAGAAAACTACAATATGAAGAAGCTTAATTAAAGTAACATTTTACATTCTTAAATTACT
TAAAATCTTCTATTATGTTTAGAACTTAACACTGCCTTACAAAATTTGAAAAGTAAATTAAAAAATATTT
AAGGATAATGAACTGCTAGTTAAAATTCTAAAAATCCATTACAAAGAAAATATTTATCTATACTGATAAA
CAACAGGGAAGAAGTGTTCGAGAAGTTATAAAGATGAAAATACTAGCCTTAAATCTGATACTTAAATCTG
CATACTTTGTTGGAGATTAAAGAGTCTATCGGGCAAATATAAGTACACTCTTCTCTTGGTATTCACAACT
GGCTGTAGCTGGAGAAAGTAGGTCTAGAAGCCCTCTCTACAAATCCAGAGCCAGCTTTGCTTTTCCTAAA
TCCTGTGCTTCTCTAATTCTTATCATGTTCTTATCATACTTATCTAATCCTTATCATCTAATTCTTATCC
CTACTTTGTAAGATAAGTACTGCCTGGACATTAGGAGGGACTAATGAAGAGTACTGTCTGTGCCACGCTT
CTTAAAACAGGATTTCCTGCAGCCCTCTGGGTGTACAGAATCTGTACAAGGATCTTTAATACAGCACAGA
CCCATGTTTTCAATTAGTTGACTCCTGCACGCCTCCTACTCTAATGCAAAACTGTTACTGGCTGTACAGA
CAGCCAATAACAAAGAACAGTAAGACCAGTCAGGCTCTGAAAGCAAAAGATAGGAAATCCAGTGGAGGGA
AAAATACTGGAGATAGCAACTTGCAGGCACAGTTTCTAAGTGTAAAATAAGTAACAGTGTTTAATCTGAC
CTAATCGATTTGCAAGAAAGATTGTGAAATGGCACGTAGCAAGAGTAGAAGGAATGGGCAGTTTGCTTTA
GAAAAATCTAACAGACTTTGGAAAAACACAAGAAAGGAAGTTCTCAACCATTCTTTCTTCATAAGAATAA
TAAACCAGATGTTCTGCCATTTGTAAACAGCTATTAACTTCACATCCTAGATCTTCATGATATTAATTTA
TCTGCTCATACAAGGGATGTTTATAAGATATTTACTGTTTCAGTTTTTATAATAGTATGCATACATATTA
AGAGATATGAGTGAATTCATTTGAGTGATTTTCTTCAAAAATGAAACCTAGTTAGTTGTTCTTACAAAGA
CAACAAAGTACATTAGCCTGCGACATCATGCTTCTCTTCAGGACAAATAAATATCGTTCTTTGAAGGTAA
GCATGTAGTAAGCATGCAGATCAAACACTGTATTAGCACCTGCTTAGAATGGCATAGAACTCATTTCTAT
CTTTACTATCCAAAAGTAATGGAAAATAGCAGTTTGGAAAGAAGACTATGGGAAAATATTTTCTTCAACT
TCTCCTCTTATCTCCTCCCACAAGGCAGATATAGCACATTTACTAGACAGTTACCTATGGCTGAATTGGC
CATTTATTTCAGTGGAAACTGCTGAAGGCAGTTCATTTTTAAAGTAAGACAAATTATTACTTTTTGATTG
ATGTAGATGTGAAAACATGCAAGAAATATTTCAAATTTTCAAGCAGTATTGATTTGTTTGGCAGACTGCA
CTATTTTCTAAAATGTTTATTTTACTTCTCAAGAGTAATGATGGCAGTAAGCTGTTTGGTGTGGTAACAT
GCCTGAGGGACAGGATGCCATCTAGAGAGACCTAGACAGACGCACGCAGTGAGCCCAGGTGAACCCCACT
TGATTCAACAAATCCAAGTGCAAGATTTTGCACCTGGTTCATGGTAACCCCCACTATCCATACAAGCTGG
AGGATGAAATGATAGAGCACAGCCCTGTTGAAAAGGACTTTTGGGGTACTGTTGAATGGCAAGCTAGACA
CGAGCCAGAAATGTGCTCTCATAGCCCAGAAAACCAACTGTATCCTGGGCTGCACCAAAAGAAGCGTGGC
CAGCAGGTCAATATATTTTGTTATTTCTATGATTCCTGATACTGATTTATTCTGCTTACTGATCTGTACT
TCTCAGCAAAATATTTTGTCAAATTCTCAGCAGCTCTGGAAATGGAAATAATTTTACTGGTTTCAATAGA
TAATGGACACACATTAAAATTTCATTCTGCTCCTTAAAAATTAAAAGTGTAGGAAAATAGTAAAATATTT
CCAGCTTCCAGATCCTTCTTGCTTATCTCGAGAATCCAGTAAATTGTAATAATTGGTATAAGATAGGGTC
ATAGGTTATAACTTTTTTTTTTTTTAATTTAAAAATGTCTCAAAATACTGCCTCACTTCACACTTCTGAT
ACTGCAATTGTTACATTTAATCTATCTATTCCAGGTATATTGCAGATCTTCATTAATATGTGATATAATT
CGCTGTCCCAGTGCTAATACTATGCTAAATCTGAGATACTGTCTAGCAGCTGCTCCTGTGGTAACTATTT
TACAAACTTAGGGTTTTCATCTTCTCCCCATCACTATAACAGACCTTACACTTTTACTTTTAAAGTTCCT
TTCTTTGACACTGTTTTTGATTTTAAAAACCTACCCCCTAATGCACAAAATCTGTAATTCTATTTTAGTT
CCCTGTTGAATATTTGTTGTCATGGAATGAAAATGTAACAAAAATATTTACAGGGACTGACTAGAAGCAT
ATATAGATCAGTACATATTTTGCTATACAAACTTCAGCACTGACTAGATATGCTGGTCCTTTTGCTATTC
TTGTTGTTTTGTTCATGTCCTTTACAATCTCTTGTGATTCAAGAGCTTACTTATACAGGAGAGTATGTTC
TGAAAAACATTTTCACTGTTTTCTGCATATTGCATCCTGAAGGGGTCTTGAGTTACACTGAGAAATTAGT
GTAAGTAGATTTAAAAGGAAAGCTCTTGATGTAAATAGTAATATTCAACTAGAAAAATCTTGCCCTAAAG
ACTTCTTTTGGGTTTCACTGCTGCTGTGACTGCAAGGTTCTTTAGAAAGGTATAAAATCATTCTCCGCAC
CAAGGACTAACTTCTTCTTAGGCCAACAGCTAAGTATAGGATCCATCTTCATAAATGATACAAGCCCAGG
CTTCAAGCATGTTTTGCAAAAGAAAGGTCAAAGGAAAGATTTAACTTCTTTCATTTCAAATTCTAATGTT
TAAGATATTGAATGGAATAATAATGATTCTGTGACCTTTTGGCAATGTTTTCAAGCAGGCATTGCAAACT
TTACTTTCTTGTTTCTTCACCTGGCATACAGGAGAAGGAAATCTTACTGGATGACTATCAAAAGCAAATG
AACAAGTATTCCTGAAAACCAAGAACATATCAGTGCATAAGTGGCAGCTAAGCAACCACACGGTTGCTTG
CTTACCATTCCCACAGTCAGATGTACAGAGGAGAAAACTGAAAGGACAGAAGTGAGGAAAACTCATAAGC
TGAGATAAAAACAGCCTAATAAATGAAAGTGAAGAATAACAGAAAGCCAAAACACAAGTGATGCAGAAGT
AATTACTTGTCACCTCCCACCAGTGGATCAATGTCCAGCCAGTTTCCAAGCAACGGCTGCTCTTTAAAAA
ATTCACCCTCTTATCCCCACCTGTGCATTTACCTACAGATTTTAGAACTTGTGATATTATATGGAATTGA
ATATGCCTTTGGGACTGCTGTCCCAGCTATGTCCCCTCTCAGCCTCTTGAAAACCCCTAGTTTATTTGCT
GGCAGGGAGGGAGAGTGAGAAACAGAGAAGGCCTTAACAGTGTAAAAAATTGTTCAGCAATGGCTAAAAT
ATTGGTGTATAATCAGCATTATTTTGGTCACAAATTTACAACTCAGCGCCGTTCAAGTAACTATGAAAAC
ACTTAAATCCATCCCAAGCAGACTCAGTACAATAGTTAAGGTATATGGAATGTGAGAGGAGAATACTGCT
GTCAATCTATTTATATCTCTGAGAGATCCCTGTGAAGGAAAGAGCTGCTGTAGACTGAGGATCAGTATTG
AAATTAAATGTTCAAATAAAGAACTTGTAGGTTCAGGATCTAAATTCTCTAAGAATAAAAATACTCACTT
CTTCCACAAAAGCAACATTGTGAGTTCCATAGTTGTACAAATATGATAAAAGCTAATGTTAGTATCTTTT
CTGTGCTGGTATTAGGTAACAAGAACCTGTATTCACTTGTAGTTGCCAAAGGTAATTGACAAAATGTATT
CAATTCTCAAGAAGATAAAATCACAGAAGGAGCAGGAACTTGTATTTTTCCATCTTGAGCTTTTCTCCTG
AAAAACACCTGGGTACACCCACTTCAGATCACTGAATGTGAAGATCACCTGGAAAATCCCCATAGGGCTA
ATATAAAGAAATCTCATTTGGGAGGAAAGTAGGCAAGGAGAAAGGACAGCATTATCTTGTACTACAGTGA
TACTGCTTCTGTGCTCAGATTCTGAAGGGGGTTCTGCTTTTGACAGAGACCTGGCTGAGAGTGCAAATGT
TTAGGTCCCAGAAGCCTGATATTAATATGGTAGAGGATGTTTGACTTAGCAGGAGAATCTTACTTCAGCA
GGGTAAAAAAATGTCTTCCCTTCCTGCACATTTTCTCAATTGAATGATAGAAAATTTTATAGTAATAAAC
TATGGACACAAACAATTAAGAATACTGGACCACTGTAGATAAAACTAACTTACACAGCAACTCAGTTTTT
CTAATTATAATTATCACATTTCAAGGATTCACTGAGAAATTTACAAACAGCACTTAATAAAGGAATTCAC
ATATACGGTAGGTAAGTGAAGTCATATAGTTTTAGCTTTCCGTAATTTCCTTTGCATGTAATTTAAATAT
TGTATAGAACTATGAATATTTTCTTTTGACTATGTTTTTGTTTTGTTTGTTTTCTTTTGTTTTGGTGACA
GCCAAAATGGCAAAATCAGCAGCTGCTTCTGTCTTATTCTGTCTTGCAAACTTTTGTCATCTGCTTAAGT
TTCTTTCTTTAAAAGAGAGAATAGTTCTACTGTGGCTTACATTTTACTCAATGATAAGTGCTTCATTGCA
ACTTGGTGATGAAAAAATTAATTTATTTTTTCAAAAGTGACAGACCATTTCTGTCTAATATAACTCTCAG
TTGCATCTTACATAAAGAAATAAATAAAAAATATTCACTTACAAAGTCTTTAATCCCTACTCTGGTGTTG
CAGGTTATCAGGTTATAACCTACAGGGGGGGGGGGGGGAATTATTGGAATATAAACTGCTTCATTTTCTT
TTCAAAGTCCAAAGTTCAACTGTGCATAAAACAAGGGCAAGTATCAAAGGCATAATAAAGTTGATTAATG
ATTAAACAGAGTAAGAAATGAAAACCTAATGCTAACATAGGGAAGGTTTTAAATAAACAAAGTGTGTTCA
AAGCAAAAGAGTAGTTTGTCAAACTAAGTAAGTGAGATAGCATTCTTAAGTTGCTGAATAGTAGTTCACA
TGAAATATTTAAGACATACAGGTAGAAAATTGCCCAGAATTGTAGAAATGATGTCTTAGGTGATCTACTG
GACCTTAAGAACCTCTTTCTTTATAAACAAATGAATTTCCCTAAATTTTACAATTAATAAAAGGTTTCTC
ACCTAAAAACTAAGTTTATTTTTCAAGTAAGGAACAATTATTCCCTTACTATTTGATGGTTGCCTACTGT
TGAAGAACAGCAAAGATTAAGACAACTACATTAATCATTAAGAACACAGCGTTCCGCTGTCATCACAGTG
AGCCCTAATGTATTAATTATGAACCAAAAACTGAAAAATGCTTCAAGATCCAATATAATTTTTGGTCTGA
CATCTTCATTAACAGCCTCCATTGACAAAAACTAGCTTGTAAAATGATCAGAACGTTTACCTATTGCAAA
ATGTTTATGATACATTTTAACTAATTTTGTTAAAATGTTTTTAAACACAAGAATGTTTTTTTTCAGCTGT
AGACAAGAAATAAGATTTAAATTTCTTAGTACATATCTAGAAATCTTCTAGAAGATTATTTCTGAATTTC
ATTTAAATTAATACATTAAAAAATATATTTAAAAAAACAGCAAAATAAGATATTGTTAAGAACTGCCAAA
TCAAGTCAAAATAAATAGAATACACATTAAAAAAAAAAAAAAGAGTTGCTTTGCTTTGTTATTTTGTTAT
TTTTTACAGAAAACAACAAATATCAGATCAAACAATGTGGAAAGGTTGTGGCCTTTCTATTGTATTCTAA
CAAAATTACTCAAGTGTTTTGACAACAGCTAAGTTGTACATATTTCATTATGAGTGCTGTTTAAAGGTGT
ATTTATTGTATTAGTACTTAACATTTTGGTTTGAATAGTCATATATTTTGGATTTCCATTTGTTATGGAA
ATAGTACAGGTTCAGCTATGTTTTTAGATATACTTTGGTTATAGGTAACTGAAATAGTTCCAAAGATGGA
TGAGATATTATTGATCTGGCCATAATTTCATTAGGTATGAAACTGAATTTACCTTTCACTTACGTACTTC
TTACATAACAGATTTTGGACACAAAAGTAATACAAGAATCTGGCACCATTTTTTGTTATTGAGTGTGGAA
ATGTATGTGATGGTGAGGTTACTATATTATCATCTCAGCAAAATCTTTTGGTTTTGGAAGCAAGACAATT
GAAATATATTGAGAGTATTTTCTGTTTAAAACAGTAATAAATACTAGTTACAAGTACTTTTCAGAGCTCC
CCTGAGGCACACACTAGTATTGCTCTCCTAGCTTTGCCAGTAGATGCTTTAATCACAGTTAATCAGCTTG
CAGCAAGTTAGCAGCAACCTTACCAATAAAGTATGGATAGACCTTTCACTCTAATTTTTTTTGCTACCAC
ACGGTGCTATCTTTTTTTTCTATTATGTTCTCTGAACAATTTTTAAACATGCACTTGTAAATTAGTGTGG
ATATAAACCACAATGAAAGAAACACAGTTTGATAAGAAATTAAACACTGGACCAAAATCGCAGTTGCAGA
TAATTAACCTACCTCTGTGAAGAAGCCGTATATGAGCTCAAATTAGTTTGATTATCAGAAATTATCACAA
ATCAAAAGTTTGTGATTCTGGAACAAAAGTTCCCAGGTTGTGCACAATAGAACAGTTAGTGTACAGCTCA
TACCTTCTCTTTTCCAGTTGTCAAACTCCAATGAAAGAATTGACAGACTTGAAAGGTCGGCCCGGGTGAA
CCTAATGAGGTTCAACACGGCAAAGTGCAGGGTTTTGCACTTGGGTTGGAGGAACCCCAGGCATCTCTAC
AGACTGGAAGGAGCAGTCCTTGAGAGCAGCTCTGCAAAAAAGGACCTGGGGGTCCTGATGGATGACAAAC
TTAACATGAGCCAGCAGTGTGCTCTTGCAGCTCGGAAAGCAAATGGTATCCTGGGCTCCATCATGAGAGG
GGTGGCCAGCAGGGACAGGGAGGTGATTGTCCCTGTCTACTCTGCTCTTGTGAGGCCCCATCTGGAGTAC
TGTGTCCAGGTATGGAGCCCCCAGTACAAGAAAGACAGAGAGCTGTTGGAGAGGGTCCAGAGAAGGGCTA
TGAAGATGATCAGCAGGCTGGAGTACCTCCCCTACGAGGACAGGCTGAGGGAGCTGGACTTATTCAGCCT
GGAGAAGACAAGGCTGCGGGGTGACCTCATTGCAGCCTTTCAGTACCTGAAGGGAGCCTATAAACAGGAA
GGGAGTAAACTCTCTGAAAGGGTAGATAACAGCAGGACAAGGGGGAACGGTTTTAAGTTGAAAGAGGGAA
GATTTAGGTTGGATGTTAGGGGGAAGTTCTTTACCAGGAGAGCGGCGAGGTGCTGGAACGGGCTGCCCAG
AGAGGTTGTGGATGCCCCATCCCTGGAGGTGTTCAAGGCCAGGCTGGATGAGGCCCTGGGCAACCCGGTC
TAGTAAATGGGGAGGTTGGTGGCCCTTCCCGGCAGGGGGGTTGGAGATTCATGATCCTTGAGGTCCCTTC
CAACCCAGGCCATTCTGTGATCTGTGAATTCTTAAATGCTTCAATCACTTCCCAGTATTAGATGCACATA
TAGTAGGTATAACACTATAAATATATACTGTATCTAACAGTTTAAGTATCTATACATGTAAATATAGCAC
TGGGCATATGTATATCTAGCACTTCACATATACACAGTCATGATATTGTAGATCATGTTACAACCGTAGG
TAATTTATAAGATTACGATGGCAAATAGGTAGCCAGGGGAAAAATCTGTAGGATAGAAAATGGAACAAAA
GATCTTTAATACTGTTCTTTCATTAAGGTAGCCTGCAGTGTGAGCAGATAGGAAAAGAGGATGAGTTTGT
AATATTACGTTGTCCGATTTTCTGCTGTTTCAGAAAATGACTACAAGCTACAGATCTTTTGGTCCTTCAT
TATAAACACAATAGGTAGCCTTGCCTTGATTGGATATCTTGTCAAATTGATCAAGTCAGTACTAAAAAAA
AAAAAATCATATGAAATAAAATTATGACACACTATCTTCTTGGTTGTATCTTTCAGAAAACACATGTGAA
AAGTATTTGTCTCCAGTACCAACTGTATCTACTTTTGAACAGCCATTTCTTTTGCCTTTTAAAGAATAAG
ACTGGACTAACCATCTTCTTCCTATGGTAACTACAAAGTTAATATTAATGAGACAGTAAATACAAATTAT
CTATTTTTTACTATTACAGTTGTTATTTGTAATGGCTTCCCATTGCCTTAGGAAGTGGATCCTCTCCTGC
CTTGTTATGTAGTCCTACAATGCTCCTCAGGCCATGGTCCCTTGCTTTATTCTTTCTTTCTCTGAGCAGG
CTCCATCCAGTTAATGTGTCTCAGCCTTTATCTCCATGATAATTCTCAAGTTGTTTGAACTGTTGCATTT
GAAGATTTCCATATGCCTCTGCAGTGAAAAAAACAATGTGAACATGTTTAAATATGTGTGCTGGTTTATG
TATATTAGATGTTTCCTTCTGCTCTGACTCCACTTTCCTGTCTTAGAAAACTGACCCTGACTCTAGCGCC
AGCATCCCCTACTTGTTCTCTCCTTGTCAAATCTCTTTACTAAGGTCTAATGTTTCTGCTCTGTCTTCCT
GGAAGAAAAAATCAGGGGTAAGTGTCCTCTGATTCTTGTTATAATACTTCTTTTATTTTTCTTTCCCAAA
AATCTTGTTATCATTCAGCCTTTGTTTAAGGCCTAAATGGTGAACAGCTGCTTGTTTTATACTAACTTCT
CAAATCAATTTCTTGTCTTCCTTTCATTGCCTTACATTACTTATAATACAGAAGTACTGAAAGGGATTGA
AATGAAAGGTCAAAATGGGGACACGCTAAAACCATGGTTCACAGTGCAATAATCTTCCAGAGGAGAAAGA
AATCTACCTCCTACCCTTTAGTGGTTCACTCCATGCAAAATATTATCCAGGGCAGGAAGAATCTTTTCTG
TACACAAAAAATTACCTGTAAAATGACATACAATATTTCTACAGGGATAAAGACAGAAATTTCATAGTAG
CAGGAGGAAAAATAATTCAGTATAAGAGAAATATACAAGAATGAAAATATACAAGAACAAAATTTGTTAT
TTTGTTGTGATACTTGGAAAGAATTGTAGGTAATTTACAATAAATAATTGATTCTCTTGCATTACGTCTT
TTAAAATAAGCTTTAAAACAAAACAAACAAACAAAAAAAAACCAACAACCTGAAAAACAACCTCATCTGT
TTAAACAGTGTGGCTCTGTGATTTTACAAGTTGTCAAATGTACTTGCTACACACATTAACACACATATAC
ATATCTTTAGTATCTGAATAATCGGTGATTCTTTTTTTCAGCATAAATAATGTGTGTACCTACTATTTAG
CCTAGTAGTCACTGTTTTTCTTCTAGTGCTTATGTACCAAAGACAGAAGCAAATCACTGTAAGTGGTCAG
ACGTTCTGAAAGATTTGGAGCTGATCAAGACATCTGAAGTAAGTTCAAAATAGTCACTATTTTATACAAT
CATAGGAGCAGACATAACTACGTGTTTAGCACAGTACTCAGCTAAGAGAGTCAGTGACCAGGAGTATGAA
ACAGGATATTGTCTGTGTAGTCATTTAGGGAATAAAAGCTAAAAGGAACTGGAGAGCTCTATTTTATAAT
AGCTCTATTTTATGTTTTTTCAGAATTTCTTTAACTGGATAAAGCAAGAAGAAACTTTTCCTAAAGAAAG
GGGCAGAGGGCATGGGAGCCCATCTTGCAGGTTACTAGGTGACTTTCAGATCACTGTCTCACTGTGTTCC
AGTGCCTGGATTAATTGAACTGCTGAACTTGAATCCATGGCTCTAGTGTTTACTTCATTATTTCTAAGTC
TGTTCATAATTACAACTCTTGTTTCCTTTTAGGACATTGATGTCAGTTTATATACTGCAAACACATACGA
GGATGTAAGTATAGTTAAAATTTGGCTCAAACTGGTCTAGTACAGTAACTTCATTTCTTTACTAGCAACT
TTTTCCAAAGGTAGACTTGCTCTAACTTGAGAAAAGCTTCAGAAACCAAATCATTTGCCTTGGAAGGCAG
AGTTCTGCAAGTTAAATTACTTATGATGAATTATGTAAGCTGGTACTAAATAATTTTACTGAAGTAGCTG
CTATTAAAATTACTCAACTAAAAGGGATGATCTAAAATGATTTTATCAAAGCAAACACTGAAAAATAATG
TTGATACTCATTTGTAATTGGAGAAAGTGAAGGAGTGACAGGGAAAATATCTACTAAGATTATTATTTAA
CACCATGCACTTGAATTCTATAATAAGAAAATGCTTGAGAAGCGCAATATGGAAAGGAACTGTCAGCATC
TGCCAATACAATTCTCTGTTTTTACAGCCTAGAAACAAATAATATGTAATATCAAGAATTTAGCCTTATA
ACTGATAGAGATTATGATTTATCCTACAAGCAATAAATTATGTTAATTTTTAATATAATTGTTAACTAAG
TCATGGTTTCCATAAACTCATTCCTGAAGAGAATTTTTCATAATTTACTGTGGAATTCAGTCTTCACTCC
ATGCAATCCTCTAAGGACATGAATCCAACCAATAACCTACTCCTAGCAGTTACTTTTAGTATAGCCCTGG
TGCAACATGAGCTGGATTCATTGCTGTGGTGATGTTGGCAGTAGCCTGCTCCTGAGACAGCCTCCTCCCG
ACAGCATAAACATTTACACAATGACTGTGCCGAATACACACAATACATTGACTCCTGTACGCAGAGTTAA
TGAGATTTCCTAGAAATAATGGACTTATTGTATCTGTGCAAAATAGTCCCAGTGTGCATGCAGAATACAG
TAACGTTGATGCATTCAGTGGACCTAGCAAGCCTGAGAAAGCAGACTCTAATGTGTCTGTGCAGTTAGCC
CTGTACACAATAGAAAAAAAAGCTGCACAGCATACATGTCCATACTCACATACCCATTCTCTTGACTGAA
ATGAGCAACTGAAATTAATACAGTATAAAGGCTAATTTATTAGCTGGTAATGTCAGGAAGATTTTCTTAA
ATTGGAAAGGCTCGATGTGAAAATCAGGCAACCTGGGGCTTGTTCTTGGTTTTATTGTTGACTTGGTGAA
AAAATGGTACCTCTGTGTTGCTGTTGTTGGTTTTTTTTAATTATTAAAATATGGGTAATATCTACTTTCT
TTTTCTCCTCCACTCTCCATTATTCTGAATAATGAACTCTGAATATAAGTGCTAGAATTACTAAGAACAC
TAATTTAATTCCATTAGTGTTAATTCTTTCCCATATTCCTATCCTAATTATTATTCCCATGTTCTTTGTC
AAGACATAGTGTTCTGCTTTGTCATAGGAAATTGGCTGGCCTATGTGTCATATATTCGCTCTTAGTATAT
TTTTGCAGATACTTAAAAGTATTCGAATAGTAAGAACAGATACTAAGTAAATAAAAATCACCACTGTCTG
GTAAATTATATCCCACACTCACTTATCCCAATTTAATCTTAAAACTGCTATAACTTCCTTATATATTTTT
TTTCCAGATAGAATGCCAGGAACCTGTAATGAGATGTTTTTTTTTAGAGATGAATGTGATTCTTCACGAA
TGTGGTATCAAAAAATGTAGTAGGAAGCATGATGTACGGAACATATTGAAAAATGGAAATGCAAGATTTG
CAACTTACCAGGTAAGTAAAACCAAAAGTCTGTGGATAAAATAGAAACGAGAGGTGTTTCTTCAGAATCA
GAAATTCTGTTTCTCATACATCTGGAAGAGGCAAGATGGTACATATGAAAGCAAAAGGGAATCAACCAGT
AACCCATGCACATAATACTGAGAAAGTTACACGTATAAAAAGGAAAGAGCAGTATTCAGTGATTTTAGAA
AAACACTCCCAGGACTCATATCAAAGAAAATAGTTGTGACTGGGCACACATGCAGACATTAATGTTTGAA
TGTGCATATATAAATAATAGTTTGTAACTTCAGTATCTAAACTCAGGTACGTTTAGTCACAAATAGAGAA
GAGTCCCAATTTTCAAAGGGATAGGTGACTTAAACCTCTCACTGAAGCACTGTATACTTGAAAGATGATC
CTATTCAGAAAACAAGCCAGGAACAAAACTTGAATACCTTGGAAGATTCCAGCAGTGCTTTCTGTAGCAA
TCGACTGCATTGGAATTACTGACAAAAACAGAATAAAAAGGGTTAGCTCTCTATCAGATCAAATAGACAA
AGTATCTTGCTTTACTATTGCATTTTCTTTTTCAGTTGAATTCCACAACATCAAAAAAATGCAAAGAATG
TGAAGAGTATGAAGAAAAAAATTTTACAGAATTTATACAGAGTTTTGTAAAGGTTATACAGAGGGAATGC
AAAAAATACATTAACTAAAATACGCAAGCTGGGAACAGTGACTTTTCAAGAAACATACCTCAGATAAATT
AGATTGTCAATAACATTGAACTAAAAAGAACTTCACTTCTAGTCCTATTTGCACAGAATAAAAAAATAAA
GAATAATAAAATCT
-
Protein Sequence : Show Sequence
>gi|45383037|ref|NP_989902.1| interleukin 15 [Gallus gallus]
MLGMAQPTQNSAGARRRPESQKTHVKSICLQYQLYLLLNSHFFCLLKNKTGLTIFFLCAYVPKTEANHCK
WSDVLKDLELIKTSEDIDVSLYTANTYEDIECQEPVMRCFFLEMKVILHECDIKKCSRKHDVRNIWKNGN
ARFATYQLNSTTAKKCKECEEYEEKNFTEFIQSFVKVIQRECKKYAN
-
Molecule Role :
Vaximmutor
- Related Vaccine(s):
E. maxima Gam82 protein vaccine
|
|
2. IL2 |
-
Gene Name :
IL2
-
Sequence Strain (Species/Organism) :
Gallus gallus
-
NCBI Gene ID :
373958
-
NCBI Protein GI :
49169785
-
Genbank Accession :
AJ224516
-
Protein Accession :
NP_989484
-
Taxonomy ID :
9031
-
Chromosome No :
4
-
Gene Starting Position :
53134198
-
Gene Ending Position :
53137243
-
Gene Strand (Orientation) :
+
-
Protein Name :
interleukin 2
-
Protein pI :
5.72
-
Protein Weight :
15833.1
-
Protein Length :
143
-
Protein Note :
Also known as IL-2
-
DNA Sequence : Show Sequence
>gi|358485508:53134198-53137243 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 4, Gallus_gallus-4.0, whole genome shotgun sequence
TATCACCTCACATTGTACATCACAATTTGAATACCAGCATACAGATAACTGGGACACTGCCATGATGTGC
AAAGTACTGATCTTTGGCTGTATTTCGGTAGCAATGCTAATGACTACAGCTTATGGAGCATCTCTATCAT
CAGCAAAAAGGAAACCTCTTCAAACATTAATAAAGGATTTAGAAATATTGGAAAATATCAAGAACGTAAG
TGGATGGTTTTCCTCTATCAGTTCTTTTCTGCTAATGTTTTCTACTTATTTTATCAATGCATTTTGTCTT
CAAAAATTAACTTTTCCTCCCTCTTCTCTACAGAAGATTCATCTCGAGCTCTACACACCAACTGAGACCC
AGGTGAGCTTTAGCCTTTTGTTTTCAGAAGGACTTCTAAGATATGGCTACTTTAAAATGATGTATTATTG
ATTTAGACCAAAACAATTCACTTATCAGAAGGGACAATATAGAAAGCAAATGTGAGGTGCTTGAATTCAG
TGTGAGGGTAGGATGGTTTCCTAAAATACACTTCTGCCTTTGATTTATATTCTTAAACATTAACAAGATA
ATGAATGATATTACTTTCTTCGTCTGTGCAATACAAAACATACAACTAATCCTCTGTATTCATTTTGCTT
ACCACACAGTTCAATTCACAAAAAAAAATGTTCTGTGAATCAATTATATGTTGTATAATATTTGCAGGAG
GCAATACTGTTGTATCAACATTAGTATAAGAACAATTCTGAGAATGAAGGGTTAGAAACTTTTTAAATGC
AGCAATATCCATAGGAAAATGCCCGTATTTTATCAAAACCAGATGCAGAGACAGTAGTTGGTTCTGTTTA
ACAAGTTACAAATCTCCCACATCCCATCAATAATGAAACTTAATTATGCTCTAACTACCTCTTTCCAGAA
GTAAAATGTAACCAACTCATCCTAGATATATTTAGCTGTAAATTCTTATTTTAAGGTGTTCTGTAAATAT
GCAAGGCTTAGTTCCATGTCAATACCGTCACTGTGGTTTCTTTTTTTTTTTTCTAAAATACTGCATACCT
GAGTCCAGGAGCATTCTAAGCACAGCATTATCACAGTCTATTTACCATTGACAGGTTGCTGACCTGAACA
CTTTTTCTAATTTAGTGTGCAAGCAGCTAGCAAGAGTATCAATTTGTTTGTTACTGGGAAGTAGTTGAGA
TACTTATGCAAGTTAACTACTTTGAGCCTTTAATTTAGTTTTTAATCCAACAGAATTCTCACATAACAGA
AATTTTGTTTGAGTAAAGATTTAAAACCAGATTCTGCAACCACTTCCTTATCTGAGTCATTTTTCTTCTA
TGCTGGCAAGATCAAGATGTAACTATCTCAGAGCTCCAGTGCTCAAATGCAGATGTGTAAGGCCAATTTA
ATAGCATACTATCACCAAGTGCCTGAAAGAGCTTATAAGAGTGATGTGTAAAGCGATGTACCCATACTCA
AGGTCAGGTAGCACACGACCCTGCCAAGGGCAAAGAGTTGGAATGAGATAGTCTATAAGGACTTCCTTCC
AACCCAAGCAATTCTGTGCTTCTATGACATTCAACTGCAAGACCAGATATAGGCATTACATAAAGGAACC
AAAGTGCATTTATTGAAGATTGCCACTGTAAAATAGAGGCTTTACTGGTAACTCTTTTCTTAATTTCCAC
ACAAAGGATTTCCACACAACTGGAAAAAATGCTCAGTTTAAGAGCTAATACAGCAGCTTCATGTTCCCAA
GAGATGTTCCATCATTTAAAATATCCTGTAAAGATTGTCTACATTTCAAAAGCTACAAGACACTAAGGCC
ACTTGTAGAATCTTATAACGTGACTTGGAGGGAAAAATTGAACTATGTACTGTATAAAAGGGAAATCAAA
TTCCTTCCTCATACAGGGAAAAAAGCAATGGGCAATTAAGAAAAAAAAAAATTCAAGTTTTTTCAGAGAA
CGTTTTATATGTCCCTACTTAAGTGTTCCCGTGTCCCTACTGCAGGAGAGACTGGCAAAAATATTTTATA
TAAATACTATATATATATTAATATGTATTGACTTTGATTACTGTATTAGGAGTGCACCCAGCAAACTCTG
CAGTGTTACCTGGGAGAAGTGGTTACTCTGAAGAAAGAAACTGAAGATGACACTGAAATTAAAGAAGAAT
TTGTAACTGCTATTCAAAATATCGAAAAGAACCTCAAGAGTCTTACGGTAGGTATTCTTTATGGTCAGTT
AGTTAAAAACAAATGTGTTTCTGCAGTAATGAAAAAGAACATCAGAATCAGTCTTTTAGTTTTGCCTTAA
GGCCTTTGTGTTATGGTTGCTTAAGAATATGGCATTTTGCACAAAAATCCTGTAATGTGAAACTACTTGA
CAACATCTACTTGCATGAAAATCCAAAACTTTAAAACAGTTCTGAGCCAAACTAACTATACAAGTCAACA
TTTTAAGAAGTATGAGATTTGTTTTCTTTGCTAAATCATGTAAGTCACCTTACAGTGCTTTTAGAAGGGT
AGCAATTATTTACATAAGTAGTAGTTCAGAAAACTATGCCATAAATTCATGACTCTAAAACTATCTGGCA
AGAGAGCTATCACCAATTAACCAAATTTATTGTACTGCAGGGTCTAAATCACACCGGAAGTGAATGCAAG
ATCTGTGAAGCTAACAACAAGAAAAAATTTCCTGATTTTCTCCATGAACTGACCAACTTTGTGAGATATC
TGCAAAAATAAGCAACTAATCATTTTTATTTTACTGCTATGTTATTTATTTAATTATTTAATTACAGATA
ATTTATATATTTTATCCCGTGGCTAACTAATCTGCTGTCCATTCTGGGACCACTGTATGCTCTTAGTCTG
GGTGATATGACGTCTGTTCTAAGATCATATTTGATCCTTTCTGTAAGCCCTACGGGCTCAAAATGTACGT
TGGAAAACTGATTGATTCTCACTTTGTCGGTAAAGTGATATGTGTTTACTGAAAGAATTTTTAAAAGTCA
CTTCTAGATGACATTTAATAAATTTCAGTAATATAT
-
Protein Sequence : Show Sequence
>gi|49169785|ref|NP_989484.1| interleukin 2 precursor [Gallus gallus]
MMCKVLIFGCISVAMLMTTAYGASLSSEKWKTLQTLIKDLEILENIKNKIHLELYTPTETQECTQQTLQC
YLGEVVTLKKETEDDTEIKEEFVTAIQNIEKNLKSLTGLNHTGSECKICEANNKKKFPDFLHELTNFVRY
LQK
-
Molecule Role :
Vaximmutor
- Related Vaccine(s):
E. maxima Gam82 protein vaccine
|
| IV. Vaccine Information |
 |
|
 |
|
|
|
|
1. CoxAbic |
| a. Tradename: |
| CoxAbic |
| b. Manufacturer: |
| Novartis AH |
| c. Vaccine Ontology ID: |
| VO_0001113 |
| d. Type: |
| Inactivated or "killed" vaccine |
| e. Status: |
| Licensed |
| f. Host Species for Licensed Use: |
| Pig |
| g. Immunization Route |
| Intramuscular injection (i.m.) |
| h. Description |
| Gametocyte antigen(s); transmission blocking through maternal antibody transfer(Wallach, 1997) |
|
|
|
 |
|
 |
|
|
|
|
|
|
|
|
|
2. E. maxima DNA vaccine pcDNA-Gam56 |
| a. Vaccine Ontology ID: |
| VO_0004527 |
| b. Type: |
| DNA vaccine |
| c. Status: |
| Research |
| d. Host Species as Laboratory Animal Model: |
| Chickens |
| e. Gene Engineering of
gam56 |
- Type:
DNA vaccine construction
- Description:
- Detailed Gene Information: Click here.
|
| f. Vector: |
| pcDNA3.1(zeo)+ (Xu et al., 2013) |
| g. Immunization Route |
| Intramuscular injection (i.m.) |
| h.
Chicken Response |
- Vaccination Protocol:
The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and 100 µg/chick). Injection was repeated once 1 week later (Xu et al., 2013).
- Vaccine Immune Response Type:
VO_0000286
- Immune Response:
Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels (Xu et al., 2013).
- Challenge Protocol:
One week after the second injection, birds were challenged orally with 5×104 sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge (Xu et al., 2013).
- Efficacy:
Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%) (Xu et al., 2013).
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
3. E. maxima Gam82 protein vaccine |
| a. Vaccine Ontology ID: |
| VO_0011467 |
| b. Type: |
| Subunit vaccine |
| c. Status: |
| Research |
| d. Antigen |
| E. maxima 82 kDa gametocyte antigen (Gam82) |
| e. Gene Engineering of
gam82 |
- Type:
Recombinant protein preparation
- Description:
The Gam82 coding sequence was amplified by PCR using Proof Start DNA polymerase (Qiagen, Valencia, CA). Amplicons were cloned into the pET28a (+) plasmid vector (Novagen/EMD Chemicals, Gibbstown, NJ) downstream from an NH2-terminal His6 epitope tag, plasmid clones were verified by sequence analysis, and used to transform competent Escherichia coli BL21 Star (Invitrogen). Eluted protein fractions were visualized on 10% SDS-acrylamide gels stained with Coomassie brilliant blue and on Western blots probed with horseradish peroxidase-conjugated anti-His monoclonal antibody (1:3000; Qiagen), and stored at −20 °C (Jang et al., 2010).
- Detailed Gene Information: Click here.
|
| f. Adjuvant: complete Freund's adjuvant |
- VO ID:
VO_0000139
- Description:
Freund's complete adjuvant (FCA)
|
| g. Immunization Route |
| Intramuscular injection (i.m.) |
| h.
Chicken Response |
- Host Strain:
Ross broiler
- Vaccination Protocol:
At 1 wk of age, chickens (30/group) were intramuscularly immunized with 100 μl of 300 or 600 μg/ml of recombinant Gam82 in Freund's complete adjuvant (FCA). Control animals received PBS in FCA. At 1 wk post-immunization, animals were given a booster injection with 100 μl of 300 or 600 μg/ml of recombinant Gam82, 3-1E, or PBS in Freund's incomplete adjuvant Ref1077:Jang et al., 2010].
- Challenge Protocol:
At 7 d post-secondary immunization, animals were given PBS or 2.0 × 10^4 E. maxima sporulated oocysts by oral gavage using an 18-gauge needle (Jang et al., 2010).
- Efficacy:
Animals vaccinated with Gam82 and challenged with E. maxima showed lower oocyst shedding and reduced intestinal pathology compared with non-vaccinated and parasite-challenged animals. Gam82 vaccination also stimulated the production of antigen-specific serum antibodies and induced greater levels of IL-2 and IL-15 mRNAs compared with non-vaccinated controls. These results demonstrate that the Gam82 recombinant protein protects against E. maxima and augments humoral and cell-mediated immunity (Jang et al., 2010).
- Host Gene Response of
IL15
- Gene Response:
Three and six days after second immunization with 60 micrograms of Gam82, there was a significant difference in IL-15 mRNA levels between the immunized and non immunized (PBS vaccinated) groups of chickens. Levels were determined in intestinal tissue (Jang et al., 2010).
- Detailed Gene Information: Click here.
- Host Gene Response of
IL2
- Gene Response:
Three days after second immunization with 60 micrograms of Gam82, there was a significant difference in IL-2 mRNA levels between the immunized and non immunized (PBS vaccinated) groups of chickens. Levels were determined in intestinal tissue (Jang et al., 2010).
- Detailed Gene Information: Click here.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
4. E. maxima oocysts with Arlacel A vaccine |
| a. Vaccine Ontology ID: |
| VO_0004229 |
| b. Type: |
| Live, attenuated vaccine |
| c. Status: |
| Research |
| d. Adjuvant: arlacel A vaccine adjuvant |
|
| e. Immunization Route |
| Intramuscular injection (i.m.) |
| f. Description |
| Chickens were vaccinated with Arlacel A injections prior to vaccination with E. maxima oocysts (Smith et al., 1994). |
| g.
Chicken Response |
- Host Strain:
Vedette
- Vaccination Protocol:
Hens were injected intramuscularly with 0.25 ml of PBS in 0.25 ml of Arlacel A in weeks 0, 2, and 6 of the study (Smith et al., 1994).
- Challenge Protocol:
At week 16 hens were infected with 20,000 oocysts of E. maxima (Smith et al., 1994).
- Efficacy:
None of the hens vaccinated had clinical symptoms of infection (Smith et al., 1994).
|
|
|
|
|
|
|
|
|
| V. References |
1. Huang et al., 2015: Huang J, Zhang Z, Li M, Song X, Yan R, Xu L, Li X. Immune protection of microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens. Avian pathology : journal of the W.V.P.A. 2015; 44(5); 392-400. [PubMed: 26181095].
2. Huang et al., 2015: Huang J, Zhang Z, Li M, Song X, Yan R, Xu L, Li X. Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge. Parasitology international. 2015; 64(5); 408-416. [PubMed: 26072304].
3. Jang et al., 2010: Jang SI, Lillehoj HS, Lee SH, Lee KW, Park MS, Cha SR, Lillehoj EP, Subramanian BM, Sriraman R, Srinivasan VA. Eimeria maxima recombinant Gam82 gametocyte antigen vaccine protects against coccidiosis and augments humoral and cell-mediated immunity. Vaccine. 2010; 28(17); 2980-2985. [PubMed: 20178868].
4. Jang et al., 2010: Jang SI, Lillehoj HS, Lee SH, Lee KW, Park MS, Cha SR, Lillehoj EP, Subramanian BM, Sriraman R, Srinivasan VA. Eimeria maxima recombinant Gam82 gametocyte antigen vaccine protects against coccidiosis and augments humoral and cell-mediated immunity. Vaccine. 2010; 28(17); 2980-2985. [PubMed: 20178868].
5. Smith et al., 1994: Smith NC, Wallach M, Miller CM, Morgenstern R, Braun R, Eckert J. Maternal transmission of immunity to Eimeria maxima: enzyme-linked immunosorbent assay analysis of protective antibodies induced by infection. Infection and immunity. 1994; 62(4); 1348-1357. [PubMed: 8132342].
6. Smith et al., 2002: Smith AL, Hesketh P, Archer A, Shirley MW. Antigenic diversity in Eimeria maxima and the influence of host genetics and immunization schedule on cross-protective immunity. Infection and immunity. 2002; 70(5); 2472-2479. [PubMed: 11953384].
7. Wallach, 1997: Wallach M. The importance of transmission-blocking immunity in the control of infections by apicomplexan parasites. International journal for parasitology. 1997; 27(10); 1159-1167. [PubMed: 9394186].
8. Witcombe et al., 2004: Witcombe DM, Ferguson DJ, Belli SI, Wallach MG, Smith NC. Eimeria maxima TRAP family protein EmTFP250: subcellular localisation and induction of immune responses by immunisation with a recombinant C-terminal derivative. International journal for parasitology. 2004; 34(7); 861-872. [PubMed: 15157769].
9. Xu et al., 2013: Xu J, Zhang Y, Tao J. Efficacy of a DNA vaccine carrying Eimeria maxima Gam56 antigen gene against coccidiosis in chickens. The Korean journal of parasitology. 2013; 51(2); 147-154. [PubMed: 23710081].
|
|
